MAP1LC3B sirna

MAP1LC3B siRNA (Rat)

Cat. Num. AAA8202436

• Gene Names: Map1lc3b; Mpl3; zbs559; Map1lc3
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAP1LC3B; MAP1LC3B siRNA (Rat); MAP1ALC3; MAP1LC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta; MAP1LC3B sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: MAP1LC3B siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 125

• Applicable Applications for MAP1LC3B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MAP1LC3B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAP1LC3B sirna

siRNA to inhibit MAP1LC3B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 41054912
• NCBI GeneID: 64862
• NCBI Accession #: NP_074058.2
• NCBI GenBank Nucleotide #: NM_022867.2
• UniProt Accession #: Q62625; Q6XVN7
• Molecular Weight: 14,599 Da
• NCBI Official Full Name: microtubule-associated proteins 1A/1B light chain 3B
• NCBI Official Synonym Full Names: microtubule-associated protein 1 light chain 3 beta
• NCBI Official Symbol: Map1lc3b
• NCBI Official Synonym Symbols: Mpl3; zbs559; Map1lc3
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3B
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3B
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: Map1lc3b
• UniProt Synonym Gene Names: Map1alc3; Map1lc3; MAP1A/MAP1B LC3 B
• UniProt Entry Name: MLP3B_RAT

NCBI Description

may mediate MAPlA and MAPlB microtubule binding activity during development of the nervous system [RGD, Feb 2006]

Uniprot Description

LC3B: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Recruits proteins such as SQSTM1 and NBR1 to the autophagosome.

Protein type: Ubiquitin-like modifier; Autophagy; Vesicle; Microtubule-binding

Cellular Component: microtubule; mitochondrion; dendrite; endomembrane system; autophagic vacuole; cytosol; microtubule associated complex; extrinsic to membrane; cell soma; membrane; axon; cytoplasm; axoneme; cytoplasmic vesicle; intracellular

Molecular Function: tubulin binding; protein domain specific binding; microtubule binding; GABA receptor binding

Biological Process: positive regulation of protein binding; mitochondrion degradation; autophagy; cellular response to nitrogen starvation; autophagic vacuole formation