KHDRBS3 sirna

KHDRBS3 siRNA (Rat)

Cat. Num. AAA8236692

• Gene Names: Khdrbs3; Etle; Slm2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KHDRBS3; KHDRBS3 siRNA (Rat); SLM2; KH domain-containing. RNA-binding. signal transduction-associated protein 3; Sam68-like mammalian protein 2; SLM-2; rSLM-2; KHDRBS3 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: KHDRBS3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 346

• Applicable Applications for KHDRBS3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KHDRBS3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KHDRBS3 sirna

siRNA to inhibit KHDRBS3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11560055
• NCBI GeneID: 64015
• NCBI Accession #: NP_071585.1
• NCBI GenBank Nucleotide #: NM_022249.1
• UniProt Accession #: Q9JLP1
• Molecular Weight: 38,779 Da
• NCBI Official Full Name: KH domain-containing, RNA-binding, signal transduction-associated protein 3
• NCBI Official Synonym Full Names: KH domain containing, RNA binding, signal transduction associated 3
• NCBI Official Symbol: Khdrbs3
• NCBI Official Synonym Symbols: Etle; Slm2
• NCBI Protein Information: KH domain-containing, RNA-binding, signal transduction-associated protein 3
• UniProt Protein Name: KH domain-containing, RNA-binding, signal transduction-associated protein 3
• Protein Family: KH domain-containing, RNA-binding, signal transduction-associated protein
• UniProt Gene Name: Khdrbs3
• UniProt Synonym Gene Names: Slm2; SLM-2; rSLM-2
• UniProt Entry Name: KHDR3_RAT

NCBI Description

mediates the selection of alternative splice sites; may provide a link between signal transduction pathways and pre-mRNA processing [RGD, Feb 2006]

Uniprot Description

KHDRBS3: RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. May play a role as a negative regulator of cell growth. Inhibits cell proliferation. Involved in splice site selection of vascular endothelial growth factor. Induces an increased concentration-dependent incorporation of exon in CD44 pre-mRNA by direct binding to purine-rich exonic enhancer. RNA- binding abilities are down-regulated by tyrosine kinase PTK6. Involved in post-transcriptional regulation of HIV-1 gene expression. Binds preferentially to the 5'-[AU]UAAA-3' motif in vitro. Belongs to the KHDRBS family. 2 isoforms of the human protein are produced by alternative splicing.

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; single-stranded RNA binding; RNA binding; SH3 domain binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; mRNA processing; positive regulation of RNA splicing