KCNMA1 sirna

KCNMA1 siRNA (Rat)

Cat. Num. AAA827870

• Gene Names: Kcnma1; Slo; BKCa; Kcnma; KCNMA1b; KCNMA1c
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KCNMA1; KCNMA1 siRNA (Rat); KCNMA; Calcium-activated potassium channel subunit alpha-1; BK channel; BKCA alpha; Calcium-activated potassium channel. subfamily M subunit alpha-1; K(VCA)alpha; KCa1.1; Maxi K channel; MaxiK; Slo-alpha; Slo1; Slowpoke homolog; Slo homolog; KCNMA1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: KCNMA1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,241

• Applicable Applications for KCNMA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KCNMA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KCNMA1 sirna

siRNA to inhibit KCNMA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13929184
• NCBI GeneID: 83731
• NCBI Accession #: NP_114016.1
• NCBI GenBank Nucleotide #: NM_031828.1
• UniProt Accession #: Q62976; O08626; O55180; O88659; Q7TMZ7; Q7TMZ8; Q7TQ55; Q7TQ56; Q7TQ57; Q9WUI3
• Molecular Weight: 138,029 Da
• NCBI Official Full Name: calcium-activated potassium channel subunit alpha-1
• NCBI Official Synonym Full Names: potassium channel, calcium activated large conductance subfamily M alpha, member 1
• NCBI Official Symbol: Kcnma1
• NCBI Official Synonym Symbols: Slo; BKCa; Kcnma; KCNMA1b; KCNMA1c
• NCBI Protein Information: calcium-activated potassium channel subunit alpha-1
• UniProt Protein Name: Calcium-activated potassium channel subunit alpha-1
• Protein Family: Calcium-activated potassium channel
• UniProt Gene Name: Kcnma1
• UniProt Synonym Gene Names: Kcnma; MaxiK; Slo homolog
• UniProt Entry Name: KCMA1_RAT

NCBI Description

large-conductance Ca(2+)-activated K(+) channel; may be involved in the shortened duration of action potential in hippocampal CA1 pyramidal neurons and in channel activity in carotid body chemoreceptor cells [RGD, Feb 2006]

Uniprot Description

KCNMA1: Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX). Defects in KCNMA1 are the cause of generalized epilepsy and paroxysmal dyskinesia (GEPD). Epilepsy is one of the most common and debilitating neurological disorders. Paroxysmal dyskinesias are neurological disorders characterized by sudden, unpredictable, disabling attacks of involuntary movement often requiring life-long treatment. The coexistence of epilepsy and paroxysmal dyskinesia in the same individual or family is an increasingly recognized phenomenon. Patients manifest absence seizures, generalized tonic-clonic seizures, paroxysmal nonkinesigenic dyskinesia, involuntary dystonic or choreiform movements. Onset is usually in childhood and patients may have seizures only, dyskinesia only, or both. Belongs to the potassium channel family. Calcium- activated (TC 1.A.1.3) subfamily. KCa1.1/KCNMA1 sub-subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Membrane protein, integral; Channel, potassium; Membrane protein, multi-pass

Cellular Component: voltage-gated potassium channel complex; endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; cell; dendrite; integral to membrane; terminal button; caveola; postsynaptic membrane; cell soma; perinuclear region of cytoplasm; cytoplasm; apical plasma membrane; plasma membrane; presynaptic active zone membrane; external side of plasma membrane

Molecular Function: protein binding; voltage-gated potassium channel activity; calcium-activated potassium channel activity; protein homodimerization activity; potassium channel activity; metal ion binding; large conductance calcium-activated potassium channel activity; protein complex binding; actin binding

Biological Process: circadian rhythm; relaxation of vascular smooth muscle; regulation of vasodilation; positive regulation of apoptosis; cell maturation; generation of action potential; auditory receptor cell differentiation; vasodilation; cellular potassium ion homeostasis; adult walking behavior; response to corticosteroid stimulus; protein homotetramerization; locomotor rhythm; synaptic transmission; sensory perception of sound; neuromuscular process controlling balance; potassium ion transport; protein homooligomerization; regulation of aldosterone metabolic process; eye blink reflex; response to osmotic stress; micturition; regulation of membrane potential; response to estrogen stimulus; smooth muscle contraction involved in micturition; response to hypoxia; response to calcium ion; negative regulation of cell volume; saliva secretion; response to pH