KCNJ4 sirna

KCNJ4 siRNA (Rat)

Cat. Num. AAA8235790

• Gene Names: Kcnj4; Hirk2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KCNJ4; KCNJ4 siRNA (Rat); IRK3; Inward rectifier potassium channel 4; BIR11; Brain inwardly rectifying K(+) channel 2; Inward rectifier K(+) channel Kir2.3; IRK-3; Potassium channel. inwardly rectifying subfamily J member 4; KCNJ4 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: KCNJ4 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 446

• Applicable Applications for KCNJ4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KCNJ4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KCNJ4 sirna

siRNA to inhibit KCNJ4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 76881824
• NCBI GeneID: 116649
• NCBI Accession #: NP_446322.2
• NCBI GenBank Nucleotide #: NM_053870.2
• UniProt Accession #: P52190; O35752
• Molecular Weight: 49,690 Da
• NCBI Official Full Name: inward rectifier potassium channel 4
• NCBI Official Synonym Full Names: potassium channel, inwardly rectifying subfamily J, member 4
• NCBI Official Symbol: Kcnj4
• NCBI Official Synonym Symbols: Hirk2
• NCBI Protein Information: inward rectifier potassium channel 4
• UniProt Protein Name: Inward rectifier potassium channel 4
• Protein Family: Inward rectifier potassium channel
• UniProt Gene Name: Kcnj4
• UniProt Synonym Gene Names: Irk3; IRK-3
• UniProt Entry Name: KCNJ4_RAT

NCBI Description

inwardly-rectifying potassium channel [RGD, Feb 2006]

Uniprot Description

HIR: This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ4 subfamily.

Protein type: Membrane protein, integral; Channel, potassium; Membrane protein, multi-pass

Cellular Component: postsynaptic membrane; cytoplasmic vesicle membrane; cell soma; integral to plasma membrane; basolateral plasma membrane; dendrite; integral to membrane; cell junction

Molecular Function: inward rectifier potassium channel activity; PDZ domain binding

Biological Process: potassium ion import