KCNA5 sirna

KCNA5 siRNA (Rat)

Cat. Num. AAA827627

• Gene Names: Kcna5; Kv1; Kv1.5
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KCNA5; KCNA5 siRNA (Rat); Potassium voltage-gated channel subfamily A member 5; RCK7; RK4; Voltage-gated potassium channel subunit Kv1.5; KCNA5 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: KCNA5 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 602

• Applicable Applications for KCNA5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KCNA5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KCNA5 sirna

siRNA to inhibit KCNA5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6981118
• NCBI GeneID: 25470
• NCBI Accession #: NP_037104.1
• NCBI GenBank Nucleotide #: NM_012972.1
• UniProt Accession #: P19024; Q6LEB7
• Molecular Weight: 66,553 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily A member 5
• NCBI Official Synonym Full Names: potassium channel, voltage gated shaker related subfamily A, member 5
• NCBI Official Symbol: Kcna5
• NCBI Official Synonym Symbols: Kv1; Kv1.5
• NCBI Protein Information: potassium voltage-gated channel subfamily A member 5
• UniProt Protein Name: Potassium voltage-gated channel subfamily A member 5
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: Kcna5
• UniProt Entry Name: KCNA5_RAT

NCBI Description

voltage gated potassium channel; functions as a delayed rectifier-like K+ channel [RGD, Feb 2006]

Uniprot Description

Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:2361015, PubMed:15618540). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (PubMed:15618540). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation (PubMed:15618540). Homotetrameric channels display rapid activation and slow inactivation. May play a role in regulating the secretion of insulin in normal pancreatic islets ().