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Immunohistochemistry (IHC) (Immunohistochemistry with Human Skin lysate tissue at an antibody concentration of 5.0ug/ml using anti-PAX7 antibody )

Rabbit PAX7 Polyclonal Antibody | anti-PAX7 antibody

PAX7 antibody - N-terminal region

Gene Names
PAX7; HUP1; RMS2; PAX7B
Reactivity
Tested Reactivity: Human, Mouse
Predicted Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
PAX7; Polyclonal Antibody; PAX7 antibody - N-terminal region; anti-PAX7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Tested Reactivity: Human, Mouse
Predicted Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml (varies by lot)
Sequence
KEEEDEADKKEDDGEKKAKHSIDGILGDKGNRLDEGSDVESEPDLPLKRK
Applicable Applications for anti-PAX7 antibody
Immunohistochemistry (IHC), Western (WB)
Protein Size (# AA)
520 amino acids
Protein Interactions
TRIM27; MYOD1; Dlg4; WDR5; ASH2L; HIRA; Ubc;
Blocking Peptide
For anti-PAX7 (MBS3200912) antibody is Catalog # MBS3225946
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human PAX7
Replacement Item
This antibody may replace item sc-130019 from Santa Cruz Biotechnology.
Predicted Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
Preparation and Storage
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry with Human Skin lysate tissue at an antibody concentration of 5.0ug/ml using anti-PAX7 antibody )

Immunohistochemistry (IHC) (Immunohistochemistry with Human Skin lysate tissue at an antibody concentration of 5.0ug/ml using anti-PAX7 antibody )

Immunohistochemistry (IHC)

(Rabbit Anti-PAX7 AntibodyParaffin Embedded Tissue: Human KidneyCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

Immunohistochemistry (IHC) (Rabbit Anti-PAX7 AntibodyParaffin Embedded Tissue: Human KidneyCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

Western Blot (WB)

(Host: RabbitTarget Name: PAX7Sample Tissue: Human OVCAR-3 Whole CellAntibody Dilution: 1ug/ml)

Western Blot (WB) (Host: RabbitTarget Name: PAX7Sample Tissue: Human OVCAR-3 Whole CellAntibody Dilution: 1ug/ml)

Western Blot (WB)

(Host: RabbitTarget Name: PAX7Sample Type: JurkatLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 0.0625ug/mLPeptide Concentration: 8.0ug/mLLysate Quantity: 25ug/laneGel Concentration: 12%)

Western Blot (WB) (Host: RabbitTarget Name: PAX7Sample Type: JurkatLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 0.0625ug/mLPeptide Concentration: 8.0ug/mLLysate Quantity: 25ug/laneGel Concentration: 12%)

Western Blot (WB)

(Lanes:1: Mouse skeletal muscle lysate, 2: Mouse skeletal muscle lysate, 3: Mouse skeletal muscle lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Anti-rabbit HRPSecondary Antibody Dilution:1:10000Gene Name:PAX7Submitted by:Anonymous)

Western Blot (WB) (Lanes:1: Mouse skeletal muscle lysate, 2: Mouse skeletal muscle lysate, 3: Mouse skeletal muscle lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Anti-rabbit HRPSecondary Antibody Dilution:1:10000Gene Name:PAX7Submitted by:Anonymous)

Western Blot (WB)

(WB Suggested Anti-PAX7 Antibody Titration: 0.05ug/mlPositive Control: Jurkat cell lysate)

Western Blot (WB) (WB Suggested Anti-PAX7 Antibody Titration: 0.05ug/mlPositive Control: Jurkat cell lysate)
Related Product Information for anti-PAX7 antibody
PAX7 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box gene 7 is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Alternative splicing in this gene has produced two known products but the biological significance of the variants is unknown.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57kDa
NCBI Official Full Name
paired box protein Pax-7 isoform 3
NCBI Official Synonym Full Names
paired box 7
NCBI Official Symbol
PAX7
NCBI Official Synonym Symbols
HUP1; RMS2; PAX7B
NCBI Protein Information
paired box protein Pax-7
UniProt Protein Name
Paired box protein Pax-7
Protein Family
UniProt Gene Name
PAX7
UniProt Synonym Gene Names
HUP1

NCBI Description

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

Transcription factor playing a role in myogenesis through regulation of muscle precursor cells proliferation.

Research Articles on PAX7

Similar Products

Product Notes

The PAX7 pax7 (Catalog #AAA3200912) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAX7 antibody - N-terminal region reacts with Tested Reactivity: Human, Mouse Predicted Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's PAX7 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western (WB). Researchers should empirically determine the suitability of the PAX7 pax7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KEEEDEADKK EDDGEKKAKH SIDGILGDKG NRLDEGSDVE SEPDLPLKRK. It is sometimes possible for the material contained within the vial of "PAX7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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