KATNA1 sirna

KATNA1 siRNA (Rat)

Cat. Num. AAA8230509

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KATNA1; KATNA1 siRNA (Rat); Katanin p60 ATPase-containing subunit A1; Katanin p60 subunit A1; p60 katanin; KATNA1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: KATNA1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 493

• Applicable Applications for KATNA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KATNA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KATNA1 sirna

siRNA to inhibit KATNA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 255708448
• NCBI GeneID: 292464
• NCBI Accession #: NP_001004217.2
• NCBI GenBank Nucleotide #: NM_001004217.4
• UniProt Accession #: Q6E0V2; Q4V7G3
• Molecular Weight: 55,844 Da
• NCBI Official Full Name: katanin p60 ATPase-containing subunit A1
• NCBI Official Synonym Full Names: katanin p60 (ATPase-containing) subunit A1
• NCBI Official Symbol: Katna1
• NCBI Protein Information: katanin p60 ATPase-containing subunit A1
• UniProt Protein Name: Katanin p60 ATPase-containing subunit A1
• UniProt Gene Name: Katna1
• UniProt Synonym Gene Names: Katanin p60 subunit A1
• UniProt Entry Name: KTNA1_RAT

NCBI Description

p60 ATPase-containing subunit of katanin, a heterodimeric enzyme (together with p80 subunit Katnb1) responsible for severing microtubules from the centrosome [RGD, Feb 2006]

Uniprot Description

Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.