KALRN sirna

KALRN siRNA (Rat)

Cat. Num. AAA8217217

• Gene Names: Kalrn; Duo; Hapip; Kalirin
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KALRN; KALRN siRNA (Rat); DUO; HAPIP; Kalirin; Huntingtin-associated protein-interacting protein; PAM COOH-terminal interactor protein 10; P-CIP10; Protein Duo; Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain; KALRN sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: KALRN siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 717

• Applicable Applications for KALRN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KALRN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KALRN sirna

siRNA to inhibit KALRN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 295054252
• NCBI GeneID: 84009
• NCBI Accession #: NP_114451.2
• NCBI GenBank Nucleotide #: NM_032062.2
• UniProt Accession #: P97924; O70135; Q6IV51; Q9JIF1; Q9JIF2; Q9JIG0; Q9JIH3
• Molecular Weight: 81,973 Da
• NCBI Official Full Name: kalirin
• NCBI Official Synonym Full Names: kalirin, RhoGEF kinase
• NCBI Official Symbol: Kalrn
• NCBI Official Synonym Symbols: Duo; Hapip; Kalirin
• NCBI Protein Information: kalirin
• UniProt Protein Name: Kalirin
• Protein Family: Kalirin
• UniProt Gene Name: Kalrn
• UniProt Synonym Gene Names: Duo; Hapip; P-CIP10
• UniProt Entry Name: KALRN_RAT

NCBI Description

binds the human huntingtin associated protein Hap1 in vitro; has GDP/GTP exchange factor activity for Rho subfamily GTP-binding proteins [RGD, Feb 2006]

Uniprot Description

kalirin: a bifunctional enzyme with protein kinase activity and guanine-exchange factor (GEF) activity. A protein kinase of the CAMK group. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity. Genetic variants are associated with susceptibility to coronary heart disease type 5 (CHDS5), a multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Interacts with the C-terminal of peptidylglycine alpha-amidating monooxygenase (PAM) and with the huntingtin-associated protein 1 (HAP1) Interacts with FASLG. The two GEF domains catalyze nucleotide exchange for RAC1 and RhoA which are bound by DH1 and DH2 respectively. The two GEF domains appear to play differing roles in neuronal development and axonal outgrowth. SH3 1 binds to the first GEF domain inhibiting GEF activity only when in the presence of a PXXP peptide, suggesting that the SH3 domain/peptide interaction mediates binding to GEF1. CRK1 SH3 domain binds to and inhibits GEF1 activity. 6 isoforms of the human protein are produced by alternative splicing. Isoform 2 is brain specific and is highly expressed in cerebral cortex, putamen, amygdala, hippocampus and caudate nucleus. Weakly expressed in brain stem and cerebellum. Isoform 4 is expressed in skeletal muscle.

Protein type: Kinase, protein; EC 2.7.11.1; GEFs; GEFs, Rac/Rho; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; CAMK group; Trio family

Cellular Component: neuron projection; cytoskeleton; cell soma; perinuclear region of cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; Rho guanyl-nucleotide exchange factor activity; enzyme binding; guanyl-nucleotide exchange factor activity; metal ion binding; ATP binding

Biological Process: nervous system development; axonogenesis; regulation of dendrite development; protein amino acid phosphorylation; positive regulation of GTPase activity