INPP4B sirna

INPP4B siRNA (Rat)

Cat. Num. AAA8211547

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: INPP4B; INPP4B siRNA (Rat); Type II inositol 3.4-bisphosphate 4-phosphatase; Inositol polyphosphate 4-phosphatase type II; INPP4B sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: INPP4B siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 942

• Applicable Applications for INPP4B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat INPP4B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for INPP4B sirna

siRNA to inhibit INPP4B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758796
• NCBI GeneID: 116699
• NCBI Accession #: NP_446369.1
• NCBI GenBank Nucleotide #: NM_053917.1
• UniProt Accession #: Q9QWG5; O35825
• Molecular Weight: 106,485 Da
• NCBI Official Full Name: type II inositol 3,4-bisphosphate 4-phosphatase
• NCBI Official Synonym Full Names: inositol polyphosphate-4-phosphatase, type II
• NCBI Official Symbol: Inpp4b
• NCBI Protein Information: type II inositol 3,4-bisphosphate 4-phosphatase
• UniProt Protein Name: Type II inositol 3,4-bisphosphate 4-phosphatase
• Protein Family: Type II inositol 3,4-bisphosphate 4-phosphatase
• UniProt Gene Name: Inpp4b
• UniProt Entry Name: INP4B_RAT

NCBI Description

enzyme that catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate, and inositol 3,4-bisphosphate [RGD, Feb 2006]

Uniprot Description

INPP4B: Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4- trisphosphate and inositol 1,4-bisphosphate. Widely expressed with highest levels occurring in the skeletal muscle and heart. Strongly inhibited by inositol hexakisphosphate. Belongs to the inositol 3,4-bisphosphate 4-phosphatase family.

Protein type: EC 3.1.3.66; Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate; Phosphatase, lipid

Cellular Component: Golgi apparatus; cell; cytoplasm

Molecular Function: phosphatidylinositol-3,4-bisphosphate 4-phosphatase activity; lipid binding

Biological Process: cellular calcium ion homeostasis; negative regulation of osteoclast differentiation; dephosphorylation; regulation of nucleocytoplasmic transport; regulation of bone remodeling; regulation of protein kinase B signaling cascade