ID3 sirna

ID3 siRNA (Rat)

Cat. Num. AAA8219516

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ID3; ID3 siRNA (Rat); ID-3; DNA-binding protein inhibitor ID-3; Inhibitor of DNA binding 3; ID3 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ID3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 119

• Applicable Applications for ID3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ID3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ID3 sirna

siRNA to inhibit ID3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6981074
• NCBI GeneID: 25585
• NCBI Accession #: NP_037190.1
• NCBI GenBank Nucleotide #: NM_013058.2
• UniProt Accession #: P41138
• Molecular Weight: 13,059 Da
• NCBI Official Full Name: DNA-binding protein inhibitor ID-3
• NCBI Official Synonym Full Names: inhibitor of DNA binding 3
• NCBI Official Symbol: Id3
• NCBI Protein Information: DNA-binding protein inhibitor ID-3
• UniProt Protein Name: DNA-binding protein inhibitor ID-3
• Protein Family: DNA-binding protein inhibitor
• UniProt Gene Name: Id3
• UniProt Synonym Gene Names: Id-3
• UniProt Entry Name: ID3_RAT

NCBI Description

helix-loop-helix transcription factor; involved in transcriptional regulation in smooth muscle cells [RGD, Feb 2006]

Uniprot Description

ID3: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors. Induced by phorbol 12-myristate 13-acetate (PMA). Interacts with COPS5 and COPS7A. Homodimer, and heterodimer with other HLH proteins.

Protein type: Transcription, coactivator/corepressor; DNA-binding

Cellular Component: nucleoplasm; microtubule cytoskeleton; cytoplasm; nucleus

Molecular Function: protein dimerization activity; protein domain specific binding; bHLH transcription factor binding; transcription factor binding; transcription factor activity

Biological Process: circadian rhythm; muscle development; central nervous system development; transcription, DNA-dependent; positive regulation of apoptosis; regulation of cell cycle; negative regulation of transcription factor activity; heart development; negative regulation of transcription from RNA polymerase II promoter; notochord development; neuron differentiation; odontogenesis; negative regulation of cell differentiation; epithelial cell differentiation; positive regulation of cell proliferation; response to wounding; negative regulation of osteoblast differentiation; negative regulation of transcription, DNA-dependent; negative regulation of myoblast differentiation; metanephros development; regulation of DNA replication