HSD17B12 sirna

HSD17B12 siRNA (Rat)

Cat. Num. AAA8218089

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HSD17B12; HSD17B12 siRNA (Rat); Estradiol 17-beta-dehydrogenase 12; 17-beta-hydroxysteroid dehydrogenase 12; 17-beta-HSD 12; 3-ketoacyl-CoA reductase; KAR; HSD17B12 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: HSD17B12 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 291

• Applicable Applications for HSD17B12 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat HSD17B12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HSD17B12 sirna

siRNA to inhibit HSD17B12 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14091750
• NCBI GeneID: 84013
• NCBI Accession #: NP_114455.1
• NCBI GenBank Nucleotide #: NM_032066.1
• UniProt Accession #: Q6P7R8; Q9Z1B9
• Molecular Weight: 34,841 Da
• NCBI Official Full Name: very-long-chain 3-oxoacyl-CoA reductase
• NCBI Official Synonym Full Names: hydroxysteroid (17-beta) dehydrogenase 12
• NCBI Official Symbol: Hsd17b12
• NCBI Protein Information: very-long-chain 3-oxoacyl-CoA reductase
• UniProt Protein Name: Very-long-chain 3-oxoacyl-CoA reductase
• Protein Family: Very-long-chain 3-oxoacyl-CoA reductase
• UniProt Gene Name: Hsd17b12
• UniProt Synonym Gene Names: KAR
• UniProt Entry Name: DHB12_RAT

NCBI Description

may play a role in steroid metabolism [RGD, Feb 2006]

Uniprot Description

HSD17B12: Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Protein type: Lipid Metabolism - unsaturated fatty acid biosynthesis; EC 1.1.1.62; Membrane protein, integral; Lipid Metabolism - androgen and estrogen; Oxidoreductase; Membrane protein, multi-pass

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; endoplasmic reticulum membrane; integral to membrane

Molecular Function: estradiol 17-beta-dehydrogenase activity; heparin binding; collagen binding; fibronectin binding; oxidoreductase activity

Biological Process: extracellular matrix organization and biogenesis; estrogen biosynthetic process; fatty acid biosynthetic process