HK1 sirna

HK1 siRNA (Rat)

Cat. Num. AAA8229211

• Gene Names: Hk1; HEXOKIN
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HK1; HK1 siRNA (Rat); Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I; HK1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: HK1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 918

• Applicable Applications for HK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat HK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HK1 sirna

siRNA to inhibit HK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6981022
• NCBI GeneID: 25058
• NCBI Accession #: NP_036866.1
• NCBI GenBank Nucleotide #: NM_012734.1
• UniProt Accession #: P05708
• Molecular Weight: 102,408 Da
• NCBI Official Full Name: hexokinase-1
• NCBI Official Synonym Full Names: hexokinase 1
• NCBI Official Symbol: Hk1
• NCBI Official Synonym Symbols: HEXOKIN
• NCBI Protein Information: hexokinase-1
• UniProt Protein Name: Hexokinase-1
• Protein Family: Probable histidine kinase
• UniProt Gene Name: Hk1
• UniProt Synonym Gene Names: HK I
• UniProt Entry Name: HXK1_RAT

NCBI Description

a ATP:D-hexose 6-phosphotransferase involved in the phosphorylation of glucose to produce glucose-6-phosphate [RGD, Feb 2006]

Uniprot Description

HK1: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). HK-2 and its mitochondrial receptor (VDAC) play the most pivotal and direct roles in the ""Warburg effect"". Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Four human isoforms are produced by alternative splicing and alternative initiation. Isoform HK1 is markedly elevated in rapidly growing tumor cells exhibiting high glucose catabolic rates. HK1 is present in most tissues but is especially prominent in brain and kidney. Isoform HK1-SC is is an integral membrane protein detected in round spermatids, condensing spermatids and mature sperm where it is found in the head membranes, mitochondria of the midpiece and the fibrous sheath of the flagellum. Isoform HK1-SA is first expressed during meiosis and continues to be present in postmeiotic germ cells while isoform HK1-SB is present only in postmeiotic germ cells.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; EC 2.7.1.1; Mitochondrial; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - starch and sucrose