D-glucuronyl C5-epimerase (GLCE) Recombinant Protein | GLCE recombinant protein

Recombinant Human D-glucuronyl C5-epimerase (GLCE), partial

Cat. Num. AAA1149096

• Gene Names: GLCE; HSEPI
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: D-glucuronyl C5-epimerase (GLCE); Recombinant Human D-glucuronyl C5-epimerase (GLCE); partial; Heparan sulfate C5-epimerase; Hsepi; Heparin/heparan sulfate:glucuronic acid C5-epimerase; Heparosan-N-sulfate-glucuronate 5-epimerase; GLCE recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 29-617aa; Partial
• Sequence: NKCSSDKAIQFPRRSSSGFRVDGFEKRAAASESNNYMNHVAKQQSEEAFPQEQQKAPPVVGGFNSNVGSKVLGLKYEEIDCLINDEHTIKGRREGNEVFLPFTWVEKYFDVYGKVVQYDGYDRFEFSHSYSKVYAQRAPYHPDGVFMSFEGYNVEVRDRVKCISGVEGVPLSTQWGPQGYFYPIQIAQYGLSHYSKNLTEKPPHIEVYETAEDRDKNKPNDWTVPKGCFMANVADKSRFTNVKQFIAPETSEGVSLQLGNTKDFIISFDLKFLTNGSVSVVLETTEKNQLFTIHYVSNAQLIAFKERDIYYGIGPRTSWSTVTRDLVTDLRKGVGLSNTKAVKPTKIMPKKVVRLIAKGKGFLDNITISTTAHMAAFFAASDWLVRNQDEKGGWPIMVTRKLGEGFKSLEPGWYSAMAQGQAISTLVRAYLLTKDHIFLNSALRATAPYKFLSEQHGVKAVFMNKHDWYEEYPTTPSSFVLNGFMYSLIGLYDLKETAGEKLGKEARSLYERGMESLKAMLPLYDTGSGTIYDLRHFMLGIAPNLARWDYHTTHINQLQLLSTIDESPVFKEFVKRWKSYLKGSRAKHN

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for GLCE recombinant protein

Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins.

Product Categories/Family for GLCE recombinant protein

Cancer

References

Homo sapiens D-glucuronyl C5-epimerase (GLCE) .Ghiselli G.Analysis of the DNA sequence and duplication history of human chromosome 15.Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.Nature 440:671-675(2006) Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.DNA Res. 5:355-364(1998) Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry.Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.J. Proteome Res. 8:651-661(2009) Using engineered 2-O-sulfotransferase to determine the activity of heparan sulfate C5-epimerase and its mutants.Li K., Bethea H.N., Liu J.J. Biol. Chem. 285:11106-11113(2010) Uncovering a biphasic catalytic mode of C5-epimerase in heparan sulfate biosynthesis.Sheng J., Xu Y., Dulaney S.B., Huang X., Liu J.J. Biol. Chem. 287:20996-21002(2012)

NCBI and Uniprot Product Information

• NCBI GI #: 51317380
• NCBI GeneID: 26035
• NCBI Accession #: NP_056369.1
• NCBI GenBank Nucleotide #: NM_015554.1
• UniProt Accession #: O94923; Q6GUQ2
• Molecular Weight: 70.9 kDa
• NCBI Official Full Name: D-glucuronyl C5-epimerase
• NCBI Official Synonym Full Names: glucuronic acid epimerase
• NCBI Official Symbol: GLCE
• NCBI Official Synonym Symbols: HSEPI
• NCBI Protein Information: D-glucuronyl C5-epimerase
• UniProt Protein Name: D-glucuronyl C5-epimerase
• Protein Family: D-glucuronyl C5-epimerase
• UniProt Gene Name: GLCE
• UniProt Synonym Gene Names: Hsepi
• UniProt Entry Name: GLCE_HUMAN

NCBI Description

Heparan sulfate (HS) is a negatively charged cell surface polysaccharide required for the biologic activities of circulating extracellular ligands. GLCE is responsible for epimerization of D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) of HS, which endows the nascent polysaccharide chain with the ability to bind growth factors and cytokines (Ghiselli and Agrawal, 2005 [PubMed 15853773]).[supplied by OMIM, Jun 2008]

Uniprot Description

GLCE: Converts D-glucuronic acid residues adjacent to N- sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins. Belongs to the D-glucuronyl C5-epimerase family.

Protein type: EC 5.1.3.17; Isomerase; Glycan Metabolism - heparan sulfate biosynthesis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q23

Cellular Component: Golgi apparatus; Golgi membrane; integral to membrane

Molecular Function: heparosan-N-sulfate-glucuronate 5-epimerase activity; racemase and epimerase activity, acting on carbohydrates and derivatives; UDP-glucuronate 5'-epimerase activity

Biological Process: carbohydrate metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan metabolic process; heparan sulfate proteoglycan biosynthetic process; heparin biosynthetic process