HDAC1 sirna

HDAC1 siRNA (Rat)

Cat. Num. AAA8200887

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HDAC1; HDAC1 siRNA (Rat); Histone deacetylase 1; HD1; HDAC1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: HDAC1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 482

• Applicable Applications for HDAC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat HDAC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HDAC1 sirna

siRNA to inhibit HDAC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 70794768
• NCBI GeneID: 297893
• NCBI Accession #: NP_001020580.1
• NCBI GenBank Nucleotide #: NM_001025409.1
• UniProt Accession #: Q4QQW4
• Molecular Weight: 55,093 Da
• NCBI Official Full Name: histone deacetylase 1
• NCBI Official Synonym Full Names: histone deacetylase 1
• NCBI Official Symbol: Hdac1
• NCBI Protein Information: histone deacetylase 1
• UniProt Protein Name: Histone deacetylase 1
• UniProt Gene Name: Hdac1
• UniProt Synonym Gene Names: HD1
• UniProt Entry Name: HDAC1_RAT

Uniprot Description

HDAC1: a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events.

Protein type: Hydrolase; EC 3.5.1.98; Nuclear receptor co-regulator

Cellular Component: nucleoplasm; heterochromatin; transcription factor complex; neuron projection; protein complex; cell soma; Sin3 complex; transcriptional repressor complex; perinuclear region of cytoplasm; nuclear chromatin; histone deacetylase complex; NuRD complex; nucleus; chromatin; cytosol

Molecular Function: deacetylase activity; nucleosomal DNA binding; transcription activator binding; histone deacetylase binding; protein deacetylase activity; protein N-terminus binding; transcription factor binding; protein binding; enzyme binding; DNA binding; protein complex binding; chromatin binding; histone deacetylase activity; transcription corepressor activity; transcription factor activity

Biological Process: circadian rhythm; positive regulation of transcription, DNA-dependent; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; response to caffeine; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of insulin secretion; neuron differentiation; negative regulation of cell proliferation; protein amino acid deacetylation; positive regulation of cell proliferation; positive regulation of oligodendrocyte differentiation; negative regulation of neuron apoptosis; circadian regulation of gene expression; response to drug; hippocampus development; response to amphetamine; histone deacetylation; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of tumor necrosis factor production; odontogenesis of dentine-containing teeth; response to hyperoxia; ATP-dependent chromatin remodeling; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; positive regulation of interleukin-1 production; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; endoderm development