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WB (Western Blot)
(Detection of human VISTA by western blot. Samples: Whole cell lysate (10 ug) from HeLa, Jurkat, HEK293T, KG-1, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody [BL-226-5C12] (AAA23805 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
VISTA recombinant antibody
Rabbit anti-VISTA Recombinant Monoclonal Antibody [BLR035F]
Gene Names
C10orf54; B7H5; GI24; B7-H5; SISP1; VISTA; PP2135; DD1alpha
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
VISTA; Monoclonal Recombinant Antibody; Rabbit anti-VISTA Recombinant Monoclonal Antibody [BLR035F]; PP2135; V-set immunoregulatory receptor; V-set domain-containing immunoregulatory receptor; sisp-1; stress-induced secreted protein-1; V-type immunoglobulin domain-containing suppressor of T-cell activation; SISP1; B7-H5; platelet receptor GI24; B7H5; C10orf54; DD1alpha; Death Domain1alpha; GI24; PD-1H; PDCD1 homolog; V-domain Ig suppressor of T cell activation; VISTA recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR035F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for VISTA recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 20 ul/mg lysate
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 ul per 1 x 10^6 cells.
IP: 20 ul/mg lysate
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 ul per 1 x 10^6 cells.
Immunogen
Between 261 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human VISTA by western blot. Samples: Whole cell lysate (10 ug) from HeLa, Jurkat, HEK293T, KG-1, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody [BL-226-5C12] (AAA23805 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human VISTA by western blot. Samples: Whole cell lysate (10 ug) from HeLa, Jurkat, HEK293T, KG-1, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody [BL-226-5C12] (AAA23805 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of human VISTA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-VISTA recombinant monoclonal antibody [BL-226-5C12] (AAA23805 lot 3) used for IP at 20 ul/mg lysate. VISTA was also immunoprecipitated by a previous lot of this antibody (AAA23805 lot 2). For blotting immunoprecipitated VISTA, AAA23805 was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human VISTA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-VISTA recombinant monoclonal antibody [BL-226-5C12] (AAA23805 lot 3) used for IP at 20 ul/mg lysate. VISTA was also immunoprecipitated by a previous lot of this antibody (AAA23805 lot 2). For blotting immunoprecipitated VISTA, AAA23805 was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human VISTA (red) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody (AAA23805) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human VISTA (red) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody (AAA23805) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human VISTA by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody (AAA23805-3). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of human VISTA by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody (AAA23805-3). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human VISTA by immunocytochemistry. Sample: FFPE section of NCI-H226 cells. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody (AAA23805-3). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human VISTA by immunocytochemistry. Sample: FFPE section of NCI-H226 cells. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody (AAA23805-3). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human VISTA in human peripheral blood mononuclear cells by flow cytometry. Antibody: Rabbit anti-VISTA recombinant monoclonal (AAA23805) (right) or isotype control (left). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human VISTA in human peripheral blood mononuclear cells by flow cytometry. Antibody: Rabbit anti-VISTA recombinant monoclonal (AAA23805) (right) or isotype control (left). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for VISTA recombinant antibody
VISTA is an immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993). It may promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). VISTA may stimulate MMP14-mediated MMP2 activation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H7M9].
Product Categories/Family for VISTA recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,908 Da
NCBI Official Full Name
platelet receptor Gi24
NCBI Official Synonym Full Names
chromosome 10 open reading frame 54
NCBI Official Symbol
C10orf54
NCBI Official Synonym Symbols
B7H5; GI24; B7-H5; SISP1; VISTA; PP2135; DD1alpha
NCBI Protein Information
platelet receptor Gi24
UniProt Protein Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
UniProt Gene Name
C10orf54
UniProt Synonym Gene Names
; Sisp-1
UniProt Entry Name
VISTA_HUMAN
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Product Notes
The VISTA c10orf54 (Catalog #AAA23805) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-VISTA Recombinant Monoclonal Antibody [BLR035F] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VISTA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the VISTA c10orf54 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VISTA, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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