GUCY2C sirna

GUCY2C siRNA (Rat)

Cat. Num. AAA8202050

• Gene Names: Gucy2c; GCC
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GUCY2C; GUCY2C siRNA (Rat); GUC2C; Heat-stable enterotoxin receptor; STA receptor; Guanylyl cyclase C; GC-C; Intestinal guanylate cyclase; GUCY2C sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: GUCY2C siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1072

• Applicable Applications for GUCY2C sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GUCY2C gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GUCY2C sirna

siRNA to inhibit GUCY2C expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6981000
• NCBI GeneID: 25711
• NCBI Accession #: NP_037302.1
• NCBI GenBank Nucleotide #: NM_013170.1
• UniProt Accession #: P23897
• Molecular Weight: 123,467 Da
• NCBI Official Full Name: heat-stable enterotoxin receptor
• NCBI Official Synonym Full Names: guanylate cyclase 2C
• NCBI Official Symbol: Gucy2c
• NCBI Official Synonym Symbols: GCC
• NCBI Protein Information: heat-stable enterotoxin receptor
• UniProt Protein Name: Heat-stable enterotoxin receptor
• Protein Family: Heat-stable enterotoxin receptor
• UniProt Gene Name: Gucy2c
• UniProt Synonym Gene Names: Guc2c; STA receptor; GC-C
• UniProt Entry Name: GUC2C_RAT

NCBI Description

guanylyl cyclase; functions as receptor for heat-stable enterotoxin responsible for acute diarrhea [RGD, Feb 2006]

Uniprot Description

GUCY2C: Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin. Defects in GUCY2C are the cause of diarrhea type 6 (DIAR6). A relatively mild, early-onset chronic diarrhea that may be associated with increased susceptibility to inflammatory bowel disease, small-bowel obstruction, and esophagitis. Defects in GUCY2C are a cause of meconium ileus (MECIL). A condition characterized by a intestinal obstruction due to inspissated meconium in the distal ileum and cecum, which develops in utero and presents shortly after birth as a failure to pass meconium. Meconium ileus is a known clinical manifestation of cystic fibrosis. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Protein type: Protein kinase, dual-specificity (receptor); Guanylyl cyclase; Nucleotide Metabolism - purine; Receptor, misc.; Kinase, protein; Protein kinase, RGC; EC 4.6.1.2; Lyase; Membrane protein, integral; RGC group; RGC family

Cellular Component: endoplasmic reticulum membrane; integral to membrane; plasma membrane

Molecular Function: toxin binding; guanylate cyclase activity; GTP binding; ATP binding; protein kinase activity

Biological Process: cGMP biosynthetic process; response to toxin; protein amino acid phosphorylation; regulation of cell proliferation