GNAQ sirna

GNAQ siRNA (Rat)

Cat. Num. AAA8218727

• Gene Names: Gnaq; Galphaq
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GNAQ; GNAQ siRNA (Rat); Guanine nucleotide-binding protein G(q) subunit alpha; Guanine nucleotide-binding protein alpha-q; GNAQ sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: GNAQ siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 359

• Applicable Applications for GNAQ sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GNAQ gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GNAQ sirna

siRNA to inhibit GNAQ expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13591957
• NCBI GeneID: 81666
• NCBI Accession #: NP_112298.1
• NCBI GenBank Nucleotide #: NM_031036.1
• UniProt Accession #: P82471
• Molecular Weight: 42,144 Da
• NCBI Official Full Name: guanine nucleotide-binding protein G(q) subunit alpha
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), q polypeptide
• NCBI Official Symbol: Gnaq
• NCBI Official Synonym Symbols: Galphaq
• NCBI Protein Information: guanine nucleotide-binding protein G(q) subunit alpha
• UniProt Protein Name: Guanine nucleotide-binding protein G(q) subunit alpha
• UniProt Gene Name: Gnaq
• UniProt Entry Name: GNAQ_RAT

NCBI Description

may mediate cardiomyocyte growth and hypertrophy; overexpression may facilitate apoptosis by inhibiting the PI3K/phosphoinositide-dependent kinase-1/Akt cell survival signaling pathway [RGD, Feb 2006]

Uniprot Description

G-alpha(q): a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Agonist binding to Gq-coupled receptors may block Akt activation via the release of active G-alpha(q) subunits that inhibit phosphatidylinositol 3-kinase. Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Protein type: G protein; G protein, heterotrimeric; G protein, heterotrimeric alpha G(q)

Cellular Component: nuclear membrane; protein complex; membrane; lysosomal membrane; plasma membrane; heterotrimeric G-protein complex; caveola; cytosol

Molecular Function: GTPase activity; protein binding; signal transducer activity; G-protein-coupled receptor binding; GTP binding; type 2A serotonin receptor binding; metal ion binding; G-protein beta/gamma-subunit binding; protein complex binding; GTPase activator activity

Biological Process: regulation of action potential; protein stabilization; metabolic process; positive regulation of apoptosis; maternal behavior; glutamate signaling pathway; positive regulation of smooth muscle cell proliferation; heart development; negative regulation of potassium ion transport; pigmentation during development; G-protein signaling, adenylate cyclase activating pathway; post-embryonic development; regulation of melanocyte differentiation; G-protein coupled receptor protein signaling pathway; forebrain neuron development; phospholipase C activation; negative regulation of protein kinase activity; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); embryonic digit morphogenesis; dopamine receptor, phospholipase C activating pathway; skeletal development; negative regulation of apoptosis; positive regulation of GTPase activity; neuron remodeling