EXOSC9 sirna

EXOSC9 siRNA (Rat)

Cat. Num. AAA8221038

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EXOSC9; EXOSC9 siRNA (Rat); Exosome complex component RRP45; Exosome component 9; EXOSC9 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: EXOSC9 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 437

• Applicable Applications for EXOSC9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat EXOSC9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EXOSC9 sirna

siRNA to inhibit EXOSC9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 70794772
• NCBI GeneID: 294975
• NCBI Accession #: NP_001020577.1
• NCBI GenBank Nucleotide #: NM_001025406.1
• UniProt Accession #: Q4QR75
• Molecular Weight: 48,882 Da
• NCBI Official Full Name: exosome complex component RRP45
• NCBI Official Synonym Full Names: exosome component 9
• NCBI Official Symbol: Exosc9
• NCBI Protein Information: exosome complex component RRP45
• UniProt Protein Name: Exosome complex component RRP45
• Protein Family: Exosome complex component
• UniProt Gene Name: Exosc9
• UniProt Entry Name: EXOS9_RAT

Uniprot Description

EXOSC9: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC9 binds to ARE-containing RNAs. Belongs to the RNase PH family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing; Ribonuclease; Nucleolus; EC 3.1.13.-

Cellular Component: nucleoplasm; nuclear chromosome; intermediate filament cytoskeleton; cytoplasm; nucleolus; exosome (RNase complex); nucleus; nuclear exosome (RNase complex)

Molecular Function: exoribonuclease activity; AU-rich element binding

Biological Process: positive regulation of cell growth; rRNA processing