DYX1C1 sirna

DYX1C1 siRNA (Rat)

Cat. Num. AAA8216472

• Gene Names: Dyx1c1; Ekn1; Edem2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DYX1C1; DYX1C1 siRNA (Rat); EKN1; Dyslexia susceptibility 1 candidate gene 1 protein homolog; DYX1C1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: DYX1C1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 420

• Applicable Applications for DYX1C1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat DYX1C1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DYX1C1 sirna

siRNA to inhibit DYX1C1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 55742717
• NCBI GeneID: 363096
• NCBI Accession #: NP_001007011.1
• NCBI GenBank Nucleotide #: NM_001007010.1
• UniProt Accession #: Q5VJS5
• Molecular Weight: 48,119 Da
• NCBI Official Full Name: dyslexia susceptibility 1 candidate gene 1 protein homolog
• NCBI Official Synonym Full Names: dyslexia susceptibility 1 candidate 1
• NCBI Official Symbol: Dyx1c1
• NCBI Official Synonym Symbols: Ekn1; Edem2
• NCBI Protein Information: dyslexia susceptibility 1 candidate gene 1 protein homolog
• UniProt Protein Name: Dyslexia susceptibility 1 candidate gene 1 protein homolog
• Protein Family: Dyslexia susceptibility 1 candidate gene 1 protein
• UniProt Gene Name: Dyx1c1
• UniProt Synonym Gene Names: Ekn1
• UniProt Entry Name: DYXC1_RAT

Uniprot Description

DYX1C1: Involved in neuronal migration during development of the cerebral neocortex. May regulate the stability and proteasomal degradation of the estrogen receptors that play an important role in neuronal differentiation, survival and plasticity. Defects in DYX1C1 may be a cause of susceptibility to dyslexia type 1 (DYX1). A relatively common, complex cognitive disorder characterized by an impairment of reading performance despite adequate motivational, educational and intellectual opportunities. It is a multifactorial trait, with evidence for familial clustering and heritability. A chromosomal aberration involving DYX1C1 has been found in a family affected by dyslexia. Translocation t(2;15)(q11;q21). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Unknown function

Cellular Component: cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; estrogen receptor binding

Biological Process: regulation of estrogen receptor signaling pathway; neuron migration; determination of left/right symmetry