DYRK1A sirna

DYRK1A siRNA (Rat)

Cat. Num. AAA8217449

• Gene Names: Dyrk1a; Dyrk; PSK47
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DYRK1A; DYRK1A siRNA (Rat); DYRK; Dual specificity tyrosine-phosphorylation-regulated kinase 1A; Dual specificity YAK1-related kinase; Protein kinase minibrain homolog; MNBH; RP86; DYRK1A sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: DYRK1A siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 754

• Applicable Applications for DYRK1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat DYRK1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DYRK1A sirna

siRNA to inhibit DYRK1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978787
• NCBI GeneID: 25255
• NCBI Accession #: NP_036923.1
• NCBI GenBank Nucleotide #: NM_012791.2
• UniProt Accession #: Q63470
• Molecular Weight: 84,513 Da
• NCBI Official Full Name: dual specificity tyrosine-phosphorylation-regulated kinase 1A
• NCBI Official Synonym Full Names: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A
• NCBI Official Symbol: Dyrk1a
• NCBI Official Synonym Symbols: Dyrk; PSK47
• NCBI Protein Information: dual specificity tyrosine-phosphorylation-regulated kinase 1A
• UniProt Protein Name: Dual specificity tyrosine-phosphorylation-regulated kinase 1A
• Protein Family: Dual specificity tyrosine-phosphorylation-regulated kinase
• UniProt Gene Name: Dyrk1a
• UniProt Synonym Gene Names: Dyrk; MNBH
• UniProt Entry Name: DYR1A_RAT

NCBI Description

catalyzes the phosphorylation of dynamin 1 and regulates dynamin 1 interaction with amphiphysin 1; may regulate assembly of the endocytic apparatus [RGD, Feb 2006]

Uniprot Description

DYRK1A: a dual-specificity protein kinase of the DYRK family. Contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. May play a significant role in a pathway regulating cell proliferation and may be involved in brain development. Its gene is localized in the Down syndrome (DS) critical region of chromosome 21, and is a strong candidate gene for learning defects associated with Down syndrome. Overexpression in mice gives rise to neurological abnormalities similar to those observed in DS. Five splice-variant isoforms have been described.

Protein type: Kinase, protein; Protein kinase, dual-specificity (non-receptor); Protein kinase, CMGC; EC 2.7.12.2; CMGC group; DYRK family; Dyrk1 subfamily

Cellular Component: nucleolus; nuclear speck; nucleus; ribonucleoprotein complex

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein self-association; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; tau protein binding; ATP binding

Biological Process: circadian rhythm; negative regulation of DNA damage response, signal transduction by p53 class mediator; nervous system development; peptidyl-serine phosphorylation; peptidyl-tyrosine phosphorylation; negative regulation of nuclear mRNA splicing, via spliceosome; protein amino acid autophosphorylation; regulation of endocytosis; peptidyl-threonine phosphorylation; protein amino acid phosphorylation; regulation of alternative nuclear mRNA splicing, via spliceosome