DRD4 sirna

DRD4 siRNA (Rat)

Cat. Num. AAA8240111

• Gene Names: Drd4; D4RA
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DRD4; DRD4 siRNA (Rat); D(4) dopamine receptor; D(2C) dopamine receptor; Dopamine D4 receptor; DRD4 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: DRD4 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 387

• Applicable Applications for DRD4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat DRD4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DRD4 sirna

siRNA to inhibit DRD4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6226851
• NCBI GeneID: 25432
• UniProt Accession #: P30729; Q62610
• Molecular Weight: 41,294 Da
• NCBI Official Full Name: D(4) dopamine receptor
• NCBI Official Synonym Full Names: dopamine receptor D4
• NCBI Official Symbol: Drd4
• NCBI Official Synonym Symbols: D4RA
• NCBI Protein Information: D(4) dopamine receptor
• UniProt Protein Name: D(4) dopamine receptor
• Protein Family: D(4) dopamine receptor
• UniProt Gene Name: Drd4
• UniProt Entry Name: DRD4_RAT

NCBI Description

may play a role in exploratory behavior and motor coordination; may be involved in lesion induced hyperactivity [RGD, Feb 2006]

Uniprot Description

DRD4: Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Its activity is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Cellular Component: cell soma; membrane; axon; integral to plasma membrane; cytoplasm; dendrite; dendritic spine; plasma membrane; terminal button; nerve terminal; vesicle membrane; cell cortex

Molecular Function: dopamine D2 receptor-like receptor activity; identical protein binding; protein binding; dopamine receptor activity; dopamine binding; drug binding; SH3 domain binding

Biological Process: circadian rhythm; fear response; synaptic transmission, dopaminergic; behavioral fear response; adult locomotory behavior; short-term memory; activation of MAPK activity; regulation of neurotransmitter secretion; positive regulation of kinase activity; regulation of calcium-mediated signaling; locomotory behavior; dopamine receptor, adenylate cyclase inhibiting pathway; regulation of inhibitory postsynaptic membrane potential; negative regulation of cAMP biosynthetic process; behavioral response to cocaine; associative learning; regulation of synaptic transmission; negative regulation of protein secretion; negative regulation of adenylate cyclase activity; response to amphetamine; regulation of dopamine metabolic process; arachidonic acid secretion; regulation of circadian rhythm; olfactory learning; response to steroid hormone stimulus; retina development in camera-type eye; photoperiodism; dopamine receptor signaling pathway; positive regulation of sodium:hydrogen antiporter activity