DBH sirna

DBH siRNA (Rat)

Cat. Num. AAA829846

• Gene Names: Dbh; DOPBHY
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DBH; DBH siRNA (Rat); Dopamine beta-hydroxylase; Dopamine beta-monooxygenase; DBH sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: DBH siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 621

• Applicable Applications for DBH sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat DBH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DBH sirna

siRNA to inhibit DBH expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 140970928
• NCBI GeneID: 25699
• NCBI Accession #: NP_037290.2
• NCBI GenBank Nucleotide #: NM_013158.2
• UniProt Accession #: Q05754
• Molecular Weight: 69,875 Da
• NCBI Official Full Name: dopamine beta-hydroxylase
• NCBI Official Synonym Full Names: dopamine beta-hydroxylase (dopamine beta-monooxygenase)
• NCBI Official Symbol: Dbh
• NCBI Official Synonym Symbols: DOPBHY
• NCBI Protein Information: dopamine beta-hydroxylase
• UniProt Protein Name: Dopamine beta-hydroxylase
• Protein Family: Dopamine beta-hydroxylase
• UniProt Gene Name: Dbh
• UniProt Entry Name: DOPO_RAT

NCBI Description

oxidoreductase that catalyzes the conversion of 3,4 dihydroxyphenethylamine, ascorbate, and O(2) to noradrenaline, dehydroascorbate, and H(2) in norephinephrine biosynthesis [RGD, Feb 2006]

Uniprot Description

DBH: Conversion of dopamine to noradrenaline. Homotetramer composed of two non-covalently bound disulfide-linked dimers. Activity is enhanced by nerve growth factor (in superior cervical ganglia and adrenal medulla). Trans-synaptic stimulation with reserpine, acetylcholine and glucocorticoids. Belongs to the copper type II ascorbate-dependent monooxygenase family.

Protein type: Membrane protein, integral; Oxidoreductase; EC 1.14.17.1; Motility/polarity/chemotaxis; Amino Acid Metabolism - tyrosine; Apoptosis; Cell cycle regulation

Cellular Component: cell soma; apical part of cell; axon; dendrite; cytoplasmic membrane-bound vesicle; cytoplasm; integral to membrane; terminal button; synapse

Molecular Function: copper ion binding; L-ascorbic acid binding; dopamine beta-monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen

Biological Process: fear response; response to peptide hormone stimulus; dopamine catabolic process; catecholamine metabolic process; response to pain; locomotory behavior; leukocyte mediated immunity; behavioral response to ethanol; glucose homeostasis; response to organic cyclic substance; response to estradiol stimulus; norepinephrine biosynthetic process; homoiothermy; visual learning; response to iron ion; associative learning; response to drug; octopamine metabolic process; sensory perception of taste; maternal behavior; response to amphetamine; multicellular organismal aging; cytokine production; social behavior; memory; regulation of cell proliferation; response to ethanol; response to copper ion; response to ozone; positive regulation of vasoconstriction; blood vessel remodeling; leukocyte migration