DAO sirna

DAO siRNA (Rat)

Cat. Num. AAA8236741

• Gene Names: Dao; Dao1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DAO; DAO siRNA (Rat); DAO1; D-amino-acid oxidase; DAAO; DAMOX; DAO sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: DAO siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 346

• Applicable Applications for DAO sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat DAO gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DAO sirna

siRNA to inhibit DAO expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758434
• NCBI GeneID: 114027
• NCBI Accession #: NP_446078.1
• NCBI GenBank Nucleotide #: NM_053626.1
• UniProt Accession #: O35078
• Molecular Weight: 38,820 Da
• NCBI Official Full Name: D-amino-acid oxidase
• NCBI Official Synonym Full Names: D-amino-acid oxidase
• NCBI Official Symbol: Dao
• NCBI Official Synonym Symbols: Dao1
• NCBI Protein Information: D-amino-acid oxidase
• UniProt Protein Name: D-amino-acid oxidase
• Protein Family: D-amino-acid oxidase
• UniProt Gene Name: Dao
• UniProt Synonym Gene Names: Dao1; DAAO; DAMOX; DAO
• UniProt Entry Name: OXDA_RAT

NCBI Description

catalyzes the conversion of d-amino acids to the corresponding alpha-keto acids; may be responsible for conversion of d-leucine to alpha-ketoisocaproic acid in the kidney [RGD, Feb 2006]

Uniprot Description

DAO: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D- amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids. Belongs to the DAMOX/DASOX family.

Protein type: Oxidoreductase; EC 1.4.3.3; Amino Acid Metabolism - glycine, serine and threonine; Other Amino Acids Metabolism - D-Arginine and D-ornithine; Amino Acid Metabolism - arginine and proline

Cellular Component: peroxisomal membrane; mitochondrial outer membrane; peroxisome; cytosol

Molecular Function: protein dimerization activity; D-amino-acid oxidase activity; cofactor binding; receptor binding

Biological Process: proline catabolic process; dopamine biosynthetic process; leucine metabolic process