CREB1 sirna

CREB1 siRNA (Rat)

Cat. Num. AAA8213381

• Gene Names: Creb1; Creb
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CREB1; CREB1 siRNA (Rat); CREB-1; Cyclic AMP-responsive element-binding protein 1; cAMP-responsive element-binding protein 1; CREB1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CREB1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 341

• Applicable Applications for CREB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CREB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CREB1 sirna

siRNA to inhibit CREB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13591924
• NCBI GeneID: 81646
• NCBI Accession #: NP_112279.1
• NCBI GenBank Nucleotide #: NM_031017.1
• UniProt Accession #: P15337
• Molecular Weight: 35,081 Da
• NCBI Official Full Name: cyclic AMP-responsive element-binding protein 1 isoform B
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 1
• NCBI Official Symbol: Creb1
• NCBI Official Synonym Symbols: Creb
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 1
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 1
• Protein Family: Cyclic AMP-responsive element-binding protein
• UniProt Gene Name: Creb1
• UniProt Synonym Gene Names: Creb-1; CREB-1; cAMP-responsive element-binding protein 1
• UniProt Entry Name: CREB1_RAT

NCBI Description

binds the cAMP response element in many gene promoters and regulates transcription [RGD, Feb 2006]

Uniprot Description

CREB: a transcription factor of the leucine zipper family of DNA binding proteins. Binds as a homodimer to the cAMP-responsive element (CRE). Phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Two splice-variant isoforms have been described.

Protein type: Transcription factor; DNA-binding

Cellular Component: nucleoplasm; transcription factor complex; mitochondrion; nuclear chromatin; chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone acetyltransferase binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; double-stranded DNA binding; transcription factor binding; transcription factor activity

Biological Process: lactation; transcription from RNA polymerase II promoter; circadian rhythm; response to glucagon stimulus; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; positive regulation of multicellular organism growth; protein amino acid phosphorylation; response to organic substance; regulation of apoptosis; mammary gland development; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; positive regulation of transforming growth factor-beta3 production; positive regulation of transcriptional preinitiation complex assembly; cell differentiation; response to drug; protein stabilization; secretory granule organization and biogenesis; positive regulation of hormone secretion; positive regulation of lipid biosynthetic process; regulation of circadian rhythm; memory; axonogenesis; pituitary gland development; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of cell size