CHRNA4 sirna

CHRNA4 siRNA (Rat)

Cat. Num. AAA8212142

• Gene Names: Chrna4; NARAC
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHRNA4; CHRNA4 siRNA (Rat); ACRA4; Neuronal acetylcholine receptor subunit alpha-4; CHRNA4 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CHRNA4 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 630

• Applicable Applications for CHRNA4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CHRNA4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHRNA4 sirna

siRNA to inhibit CHRNA4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14149758
• NCBI GeneID: 25590
• NCBI Accession #: NP_077330.1
• NCBI GenBank Nucleotide #: NM_024354.1
• UniProt Accession #: P09483; O35769
• Molecular Weight: 70,320 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit alpha-4
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, alpha 4 (neuronal)
• NCBI Official Symbol: Chrna4
• NCBI Official Synonym Symbols: NARAC
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-4
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-4
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: Chrna4
• UniProt Synonym Gene Names: Acra4
• UniProt Entry Name: ACHA4_RAT

NCBI Description

nicotinic acetylcholine receptor subunit responsible for binding calcium sensor protein visinin-like protein-1 (Vilip1), which modulates acetylcholine receptor expression and sensitivity [RGD, Feb 2006]

Uniprot Description

nAChRA4: a ligand-gated ion channels that binds acetylcholine and mediates fast signal transmission at synapses. After binding acetylcholine, these pentameric receptors undergo an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. An integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor.

Protein type: Receptor, misc.; Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; membrane; cell soma; dendrite; plasma membrane; cell junction; external side of plasma membrane

Molecular Function: protein binding; protein heterodimerization activity; acetylcholine receptor activity; ion channel activity; drug binding; acetylcholine binding; nicotinic acetylcholine-activated cation-selective channel activity

Biological Process: response to nicotine; positive regulation of dopamine secretion; regulation of action potential; B cell activation; protein heterooligomerization; behavioral response to nicotine; locomotory behavior; respiratory gaseous exchange; signal transduction; sensory perception of pain; DNA repair; regulation of dopamine secretion; synaptic transmission, cholinergic; neurological system process; membrane depolarization; regulation of inhibitory postsynaptic membrane potential; regulation of membrane potential; calcium ion transport; response to hypoxia; response to oxidative stress; cognition; cation transport