CEBPG sirna

CEBPG siRNA (Rat)

Cat. Num. AAA828586

• Gene Names: Cebpg; C/EBP; CEBPRNA
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CEBPG; CEBPG siRNA (Rat); CCAAT/enhancer-binding protein gamma; C/EBP gamma; CEBPG sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CEBPG siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 150

• Applicable Applications for CEBPG sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CEBPG gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CEBPG sirna

siRNA to inhibit CEBPG expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148224453
• NCBI GeneID: 25301
• NCBI Accession #: NP_036963.1
• NCBI GenBank Nucleotide #: NM_012831.1
• UniProt Accession #: P26801
• Molecular Weight: 16,388 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein gamma
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), gamma
• NCBI Official Symbol: Cebpg
• NCBI Official Synonym Symbols: C/EBP; CEBPRNA
• NCBI Protein Information: CCAAT/enhancer-binding protein gamma
• UniProt Protein Name: CCAAT/enhancer-binding protein gamma
• Protein Family: CCAAT/enhancer-binding protein
• UniProt Gene Name: Cebpg
• UniProt Synonym Gene Names: C/EBP gamma
• UniProt Entry Name: CEBPG_RAT

NCBI Description

binds to the promoter and enhancer of the alpha 1-fetoprotein gene; may regulate alpha 1-fetoprotein transcription [RGD, Feb 2006]

Uniprot Description

C/EBP-gamma: Transcription factor that binds to the enhancer element PRE-I (positive regulatory element-I) of the IL-4 gene. Might change the DNA-binding specificity of other transcription factors and recruit them to unusual DNA sites. Belongs to the bZIP family. C/EBP subfamily.

Protein type: DNA repair, damage; Apoptosis; Transcription factor; Transcription, coactivator/corepressor; DNA-binding; Motility/polarity/chemotaxis

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; double-stranded DNA binding; transcription factor activity; transcription factor binding

Biological Process: positive regulation of DNA repair; transcription, DNA-dependent; natural killer cell mediated cytotoxicity; negative regulation of transcription factor activity; enucleate erythrocyte differentiation; liver development; mRNA metabolic process; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of interferon-gamma biosynthetic process