CD44 sirna

CD44 siRNA (Rat)

Cat. Num. AAA8236040

• Gene Names: Cd44; CD44A; METAA; RHAMM
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CD44; CD44 siRNA (Rat); CD44 antigen; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; PGP-I; CD antigen CD44; CD44 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CD44 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 364

• Applicable Applications for CD44 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CD44 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CD44 sirna

siRNA to inhibit CD44 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 564342041
• NCBI GeneID: 25406
• NCBI Accession #: XP_006234693.1
• NCBI GenBank Nucleotide #: XM_006234631.2
• UniProt Accession #: P26051; Q99021
• Molecular Weight: 39,794 Da
• NCBI Official Full Name: CD44 antigen isoform X7
• NCBI Official Synonym Full Names: CD44 molecule (Indian blood group)
• NCBI Official Symbol: Cd44
• NCBI Official Synonym Symbols: CD44A; METAA; RHAMM
• NCBI Protein Information: CD44 antigen
• UniProt Protein Name: CD44 antigen
• Protein Family: CD44 antigen
• UniProt Gene Name: Cd44
• UniProt Synonym Gene Names: ECMR-III; PGP-1; PGP-I
• UniProt Entry Name: CD44_RAT

NCBI Description

adhesion molecule involved in migration, cell fusion and resportion in osteoclasts that also plays a role in cellular metastasis [RGD, Feb 2006]

Uniprot Description

CD44: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Interacts with PKN2. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. 19 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc.

Cellular Component: Golgi apparatus; focal adhesion; cell surface; protein complex; basolateral plasma membrane; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; nucleus; external side of plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; transmembrane receptor activity; cytokine binding; hyaluronic acid binding; phosphoprotein binding; protein kinase binding; epidermal growth factor receptor binding; hyalurononglucosaminidase activity

Biological Process: cell migration; positive regulation of heterotypic cell-cell adhesion; Wnt receptor signaling pathway; positive regulation of neutrophil apoptosis; negative regulation of caspase activity; blood vessel maturation; response to organic cyclic substance; positive regulation of peptidyl-serine phosphorylation; hyaluronan catabolic process; response to vitamin A; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of mature B cell apoptosis; ureteric bud branching; cartilage development; regulation of cell growth; inflammatory response; cell adhesion; healing during inflammatory response; neurite development; negative regulation of apoptosis