CASP2 sirna

CASP2 siRNA (Rat)

Cat. Num. AAA8208632

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CASP2; CASP2 siRNA (Rat); ICH1; Caspase-2; CASP-2; Protease ICH-1; CASP2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CASP2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 452

• Applicable Applications for CASP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CASP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CASP2 sirna

siRNA to inhibit CASP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19923687
• NCBI GeneID: 64314
• NCBI Accession #: NP_071967.2
• NCBI GenBank Nucleotide #: NM_022522.2
• UniProt Accession #: P55215; O35398; O55194; Q9WUI6
• Molecular Weight: 38,004 Da
• NCBI Official Full Name: caspase-2
• NCBI Official Synonym Full Names: caspase 2
• NCBI Official Symbol: Casp2
• NCBI Protein Information: caspase-2
• UniProt Protein Name: Caspase-2
• Protein Family: Caspase
• UniProt Gene Name: Casp2
• UniProt Synonym Gene Names: Ich1; CASP-2
• UniProt Entry Name: CASP2_RAT

NCBI Description

may play a role in proteolysis and programmed cell death [RGD, Feb 2006]

Uniprot Description

CASP2: Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Interacts with LRDD. Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.55; Apoptosis; Protease

Cellular Component: membrane; mitochondrion; cytoplasm; nucleus

Molecular Function: protein domain specific binding; protein binding; cysteine-type endopeptidase activity

Biological Process: luteolysis; positive regulation of apoptosis; apoptosis; germ cell programmed cell death; programmed cell death; proteolysis; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of neuron apoptosis; protein processing; brain development; aging