CAPN3 sirna

CAPN3 siRNA (Rat)

Cat. Num. AAA8220633

• Gene Names: Capn3; p94; Lp82; Lp84; Lp85
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CAPN3; CAPN3 siRNA (Rat); Calpain-3; Calcium-activated neutral proteinase 3; CANP 3; Calpain L3; Calpain p94; Muscle-specific calcium-activated neutral protease 3; CAPN3 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CAPN3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 821

• Applicable Applications for CAPN3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CAPN3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CAPN3 sirna

siRNA to inhibit CAPN3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8393041
• NCBI GeneID: 29155
• NCBI Accession #: NP_058813.1
• NCBI GenBank Nucleotide #: NM_017117.1
• UniProt Accession #: P16259
• Molecular Weight: 94,127 Da
• NCBI Official Full Name: calpain-3
• NCBI Official Synonym Full Names: calpain 3
• NCBI Official Symbol: Capn3
• NCBI Official Synonym Symbols: p94; Lp82; Lp84; Lp85
• NCBI Protein Information: calpain-3
• UniProt Protein Name: Calpain-3
• Protein Family: Calpain
• UniProt Gene Name: Capn3
• UniProt Synonym Gene Names: CANP 3
• UniProt Entry Name: CAN3_RAT

NCBI Description

calcium-activated protease; alternative splicing produces different protein products; may be involved in astrocyte plasticity and in eye development and pathology [RGD, Feb 2006]

Uniprot Description

CAPN3: Calcium-regulated non-lysosomal thiol-protease. Defects in CAPN3 are the cause of limb-girdle muscular dystrophy type 2A (LGMD2A). LGMD2A is an autosomal recessive degenerative myopathy characterized by progressive symmetrical atrophy and weakness of the proximal limb muscles and elevated serum creatine kinase. The symptoms usually begin during the first two decades of life, and the disease gradually worsens, often resulting in loss of walking ability 10 or 20 years after onset. Belongs to the peptidase C2 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding; Motility/polarity/chemotaxis; Protease; EC 3.4.22.54; Cell development/differentiation

Cellular Component: protein complex; myofibril; T-tubule; cytoplasm; plasma membrane; Z disc; cytosol; nucleus

Molecular Function: peptidase activity; protein binding; sodium ion binding; enzyme binding; structural constituent of muscle; titin binding; calcium-dependent cysteine-type endopeptidase activity; protein complex scaffold; calcium ion binding; catalytic activity; ligase regulator activity; receptor binding

Biological Process: response to drug; response to muscle activity; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; positive regulation of satellite cell activation involved in skeletal muscle regeneration; sarcomere organization; proteolysis; negative regulation of protein sumoylation; activation of NF-kappaB transcription factor; autolysis; myofibril assembly; regulation of catalytic activity; regulation of myoblast differentiation; protein complex assembly; positive regulation of release of sequestered calcium ion into cytosol; response to calcium ion; negative regulation of transcription, DNA-dependent; regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of apoptosis