CAMK2D sirna

CAMK2D siRNA (Rat)

Cat. Num. AAA8233189

• Gene Names: Camk2d; CAMK1; Camk2; Camki; RATCAMKI
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CAMK2D; CAMK2D siRNA (Rat); Calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II subunit delta; CaMK-II subunit delta; CAMK2D sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CAMK2D siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 533

• Applicable Applications for CAMK2D sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CAMK2D gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CAMK2D sirna

siRNA to inhibit CAMK2D expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978595
• NCBI GeneID: 24246
• NCBI Accession #: NP_036651.1
• NCBI GenBank Nucleotide #: NM_012519.2
• UniProt Accession #: P15791; P97915; P97916; Q3B7L0; Q63904; Q63905; Q63906; Q63907; Q63908
• Molecular Weight: 56,293 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit delta
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II delta
• NCBI Official Symbol: Camk2d
• NCBI Official Synonym Symbols: CAMK1; Camk2; Camki; RATCAMKI
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit delta
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit delta
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: Camk2d
• UniProt Synonym Gene Names: CaM kinase II subunit delta; CaMK-II subunit delta
• UniProt Entry Name: KCC2D_RAT

NCBI Description

subunit of calcium/calmodulin dependent protein kinase II; regulates potassium currents in brain capillary endothelial cells under hypoxic conditions; expressed as different isoforms in different tissues [RGD, Feb 2006]

Uniprot Description

CAMK2D: a protein kinase of the calcium/calmodulin-dependent protein kinase (CAMK) group that interacts with the sarcoplasmic reticulum membrane in cardiac and skeletal muscle. Regulates Ca2+ homeostatis and excitation-contraction coupling (ECC) in heart. Calmodulin binding induces conformational changes that relieve autoinhibition, permitting autophosphorylation at T287, rendering the kinase constitutively active and independent of Ca2+-binding. Targets ion channels, transporters and accessory proteins involved in Ca2+ influx into the myocyte, Ca2+ release from the sarcoplasmic reticulum (SR), SR Ca2+ uptake and Na+ and K+ channel transport. Also targets transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca2+ release via direct phosphorylation of RYR2 Ca2+ channel on S2808. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca2+ influx to myocytes by binding and phosphorylating the L-type Ca2+ channel subunit beta-2 CACNB2. In addition to Ca2+ channels, can target and regulate the cardiac sarcolemmal Na+ channel SCN5A and the K+ channel Kv4.3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLB), an endogenous inhibitor of SERCA2A, contributing to the enhancement of SR Ca2+ uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca2+ transport through phosphorylation of PLB and TRDN, a ryanodine receptor-coupling factor. CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with RRAD and CACNB2. Ten isoforms of the human protein are produced by alternative splicing. Isoform Delta3, isoform Delta2, isoform Delta8 and isoform Delta9 are expressed in cardiac muscle. Isoform Delta11 is expressed in skeletal muscle. Activity is induced in skeletal muscle during exercise. The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.17; Protein kinase, CAMK; CAMK group; CAMK2 family

Cellular Component: sarcoplasmic reticulum membrane; protein complex; cell soma; membrane; sarcoplasmic reticulum; perinuclear region of cytoplasm; T-tubule; cytoplasm; cytosol; neuromuscular junction; nucleus; sarcolemma

Molecular Function: calmodulin binding; sodium channel inhibitor activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; titin binding; nitric-oxide synthase binding; ATP binding

Biological Process: positive regulation of smooth muscle cell proliferation; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; regulation of sodium ion transport; positive regulation of smooth muscle cell migration; protein amino acid phosphorylation; cellular potassium ion homeostasis; protein oligomerization; positive regulation of Rac protein signal transduction; endoplasmic reticulum calcium ion homeostasis; peptidyl-serine phosphorylation; calcium ion transport; regulation of G2/M transition of mitotic cell cycle; response to hypoxia; cardiac muscle contraction; G1/S transition of mitotic cell cycle