BMP2 sirna

BMP2 siRNA (Rat)

Cat. Num. AAA8207996

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BMP2; BMP2 siRNA (Rat); BMP-2; Bone morphogenetic protein 2; Bone morphogenetic protein 2A; BMP-2A; BMP2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: BMP2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 393

• Applicable Applications for BMP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat BMP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BMP2 sirna

siRNA to inhibit BMP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8392990
• NCBI GeneID: 29373
• NCBI Accession #: NP_058874.1
• NCBI GenBank Nucleotide #: NM_017178.1
• UniProt Accession #: P49001
• Molecular Weight: 44,383 Da
• NCBI Official Full Name: bone morphogenetic protein 2
• NCBI Official Synonym Full Names: bone morphogenetic protein 2
• NCBI Official Symbol: Bmp2
• NCBI Protein Information: bone morphogenetic protein 2
• UniProt Protein Name: Bone morphogenetic protein 2
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: Bmp2
• UniProt Synonym Gene Names: Bmp-2; BMP-2; BMP-2A
• UniProt Entry Name: BMP2_RAT

NCBI Description

involved in cellular signaling during limb development; induces bone formation [RGD, Feb 2006]

Uniprot Description

BMP2: Induces cartilage and bone formation. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Cellular Component: extracellular space; protein complex; cell surface; cytoplasm; extracellular region

Molecular Function: protein domain specific binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; phosphatase activator activity; cytokine activity; SMAD binding; transforming growth factor beta receptor binding; receptor binding; retinol dehydrogenase activity

Biological Process: activation of MAPK activity; positive regulation of apoptosis; heart development; positive regulation of transcription, DNA-dependent; adrenocorticotropin hormone secreting cell differentiation; telencephalon regionalization; protein amino acid phosphorylation; cardiac muscle cell differentiation; regulation of apoptosis; BMP signaling pathway; ovulation cycle; chondrocyte differentiation; inner ear development; regulation of odontogenesis of dentine-containing teeth; positive regulation of astrocyte differentiation; pericardium development; positive regulation of neurogenesis; cell fate commitment; organ morphogenesis; mesenchymal cell differentiation; response to mechanical stimulus; regulation of MAPKKK cascade; positive regulation of osteoblast proliferation; positive regulation of fat cell differentiation; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; negative regulation of transcription, DNA-dependent; negative regulation of calcium-independent cell-cell adhesion; positive regulation of odontogenesis; proteoglycan metabolic process; embryonic heart tube anterior/posterior pattern formation; negative regulation of insulin-like growth factor receptor signaling pathway; thyroid stimulating hormone secreting cell differentiation; negative regulation of transcription from RNA polymerase II promoter; bone mineralization; negative regulation of cell cycle; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; regulation of transcription, DNA-dependent; cardiac muscle morphogensis; negative regulation of aldosterone biosynthetic process; inflammatory response; positive regulation of Wnt receptor signaling pathway; cardiac cell differentiation; ossification; Notch signaling pathway; response to retinoic acid; protein destabilization; in utero embryonic development; positive regulation of bone mineralization; positive regulation of ossification; odontogenesis of dentine-containing teeth; osteoblast differentiation; positive regulation of osteoblast differentiation; telencephalon development; ureteric bud branching; cartilage development; response to hypoxia; epithelial to mesenchymal transition; positive regulation of protein amino acid phosphorylation; positive regulation of neuron differentiation; growth; positive regulation of cell migration