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AP2M1 sirna

AP2M1 siRNA (Rat)

Gene Names
Ap2m1; mu2; Ap50; Clapm1
Reactivity
Rat
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
AP2M1; AP2M1 siRNA (Rat); AP-2 complex subunit mu; AP-2 mu chain; Adapter-related protein complex 2 subunit mu; Adaptor protein complex AP-2 subunit mu; Clathrin assembly protein complex 2 mu medium chain; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; Mu2-adaptin; Plasma membrane adaptor AP-2 50 kDa protein; AP2M1 sirna
Ordering
For Research Use Only!
Host
Synthetic
Reactivity
Rat
Specificity
AP2M1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
435
Applicable Applications for AP2M1 sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat AP2M1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for AP2M1 sirna
siRNA to inhibit AP2M1 expression using RNA interference

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,655 Da
NCBI Official Full Name
AP-2 complex subunit mu
NCBI Official Synonym Full Names
adaptor-related protein complex 2, mu 1 subunit
NCBI Official Symbol
Ap2m1
NCBI Official Synonym Symbols
mu2; Ap50; Clapm1
NCBI Protein Information
AP-2 complex subunit mu
UniProt Protein Name
AP-2 complex subunit mu
UniProt Gene Name
Ap2m1
UniProt Entry Name
AP2M1_RAT

NCBI Description

encodes the mu subunit of the heterotetrameric coat assembly protein complex 2 (AP2); associated with clathrin-coated vesicles and may be important for intracellular trafficking [RGD, Feb 2006]

Uniprot Description

AP2M1: a protein of the adaptor complexes medium subunit family. A subunit of the heterotetrameric coat assembly protein complex 2 (AP2) which links clathrin to receptors in coated vesicles. Interacts with the cytoplasmic tails of membrane proteins and polyphosphoinositide-containing lipids. Is not required for clathrin-coated vesicle formation at the plasma membrane, but that it is one of several endocytic adaptors required for the uptake of certain cargo proteins. Is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP-2 is a heterotetramer composed of two large chains (alpha and beta), a medium chain (AP50) and a small chain (AP17).

Protein type: Vesicle; Adaptor/scaffold

Cellular Component: clathrin vesicle coat; transport vesicle; clathrin adaptor complex; mitochondrion; plasma membrane; terminal button; coated pit

Molecular Function: protein binding; low-density lipoprotein receptor binding; signal sequence binding; lipid binding

Biological Process: vesicle-mediated transport; intracellular protein transport; endocytosis

Research Articles on AP2M1

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Product Notes

The AP2M1 ap2m1 (Catalog #AAA8238493) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The AP2M1 siRNA (Rat) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AP2M1 can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the AP2M1 ap2m1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AP2M1, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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