ALDOA sirna

ALDOA siRNA (Rat)

Cat. Num. AAA8238276

• Gene Names: Aldoa; Aldo1; RNALDOG5
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ALDOA; ALDOA siRNA (Rat); Fructose-bisphosphate aldolase A; Muscle-type aldolase; ALDOA sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ALDOA siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 364

• Applicable Applications for ALDOA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ALDOA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ALDOA sirna

siRNA to inhibit ALDOA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 293336190
• NCBI GeneID: 24189
• NCBI Accession #: NP_001170776.1
• NCBI GenBank Nucleotide #: NM_001177305.1
• UniProt Accession #: P05065; Q63038
• Molecular Weight: 39,352 Da
• NCBI Official Full Name: fructose-bisphosphate aldolase A
• NCBI Official Synonym Full Names: aldolase A, fructose-bisphosphate
• NCBI Official Symbol: Aldoa
• NCBI Official Synonym Symbols: Aldo1; RNALDOG5
• NCBI Protein Information: fructose-bisphosphate aldolase A
• UniProt Protein Name: Fructose-bisphosphate aldolase A
• Protein Family: Fructose-bisphosphate aldolase
• UniProt Gene Name: Aldoa
• UniProt Entry Name: ALDOA_RAT

NCBI Description

member of the aldolase enzyme family [RGD, Feb 2006]

Uniprot Description

ALDOA: a glycolytic enzyme that catalyzes D-fructose 1,6-bisphosphate -> glycerone phosphate + D-glyceraldehyde 3-phosphate. Three forms of aldolase are found in vertebrates - aldolase A in muscle, aldolase B in liver and aldolase C in brain.

Protein type: Lyase; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.1.2.13; Mitochondrial; Carbohydrate Metabolism - fructose and mannose

Cellular Component: extracellular space; protein complex; mitochondrion; membrane; cytoplasm; M band; nucleus; Z disc; actin cytoskeleton

Molecular Function: protein binding; protease binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity

Biological Process: response to nicotine; muscle maintenance; regulation of cell shape; fructose 1,6-bisphosphate metabolic process; glycolysis; striated muscle contraction; response to heat; response to estrogen stimulus; ATP biosynthetic process; response to hypoxia; response to lipopolysaccharide; fructose metabolic process; protein homotetramerization