AGXT sirna

AGXT siRNA (Rat)

Cat. Num. AAA8216940

• Gene Names: Agxt; AGT; SPT; Spat
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AGXT; AGXT siRNA (Rat); AGT1; Serine--pyruvate aminotransferase mitochondrial; SPT; Alanine--glyoxylate aminotransferase; AGT; AGXT sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: AGXT siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 392

• Applicable Applications for AGXT sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat AGXT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AGXT sirna

siRNA to inhibit AGXT expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 452881613
• NCBI GeneID: 24792
• NCBI Accession #: NP_001263635.1
• NCBI GenBank Nucleotide #: NM_001276706.1
• UniProt Accession #: P09139; Q9R2C7
• Molecular Weight: 43,399 Da
• NCBI Official Full Name: serine--pyruvate aminotransferase, peroxisomal isoform 3
• NCBI Official Synonym Full Names: alanine-glyoxylate aminotransferase
• NCBI Official Symbol: Agxt
• NCBI Official Synonym Symbols: AGT; SPT; Spat
• NCBI Protein Information: serine--pyruvate aminotransferase; serine--pyruvate aminotransferase, mitochondrial; serine--pyruvate aminotransferase, peroxisomal
• UniProt Protein Name: Serine--pyruvate aminotransferase, mitochondrial
• Protein Family: Serine--pyruvate aminotransferase
• UniProt Gene Name: Agxt
• UniProt Synonym Gene Names: Agt1; SPT; AGT
• UniProt Entry Name: SPYA_RAT

NCBI Description

This gene encodes alanine-glyoxylate aminotransferase, which catalyzes the interconversion of L-alanine and glyoxylate to pyruvate and glycine. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. The longer transcript variant includes an upstream translation start codon and a downstream translation start codon. The upstream start codon initiates the translation of the mitochondrial enzyme precursor while the downstream start codon initiates the translation of the peroxisomal enzyme (see PMID:2332438). [provided by RefSeq, Feb 2013]

Uniprot Description

AGXT: Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1); also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Transferase; Mitochondrial; Amino Acid Metabolism - glycine, serine and threonine; EC 2.6.1.51; EC 2.6.1.44; Amino Acid Metabolism - alanine, aspartate and glutamate; Motility/polarity/chemotaxis

Cellular Component: peroxisomal matrix; mitochondrion; mitochondrial matrix; peroxisome

Molecular Function: amino acid binding; protein homodimerization activity; alanine-glyoxylate transaminase activity; serine-pyruvate transaminase activity; transaminase activity; pyridoxal phosphate binding; receptor binding

Biological Process: pyruvate biosynthetic process; response to cAMP; glyoxylate catabolic process; glyoxylate metabolic process; glycine biosynthetic process, by transamination of glyoxylate; response to glucocorticoid stimulus; oxalic acid secretion; L-cysteine catabolic process; L-alanine catabolic process