DARPP32 Blocking Peptide | PPP1R1B blocking peptide

DARPP32 (pT34) Blocking Peptide

Cat. Num. AAA8243674

• Gene Names: PPP1R1B; DARPP32; DARPP-32
• Reactivity: Human, Mouse, Bovine, Pig
• Applications: Blocking (BL)
• Purity: >85%
• Synonyms: DARPP32; DARPP32 (pT34) Blocking Peptide; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein; PPP1R1B blocking peptide

• Host: Synthetic
• Reactivity: Human, Mouse, Bovine, Pig
• Purity/Purification: >85%
• Form/Format: Lyophilized Powder
• Sequence Length: 204

• Applicable Applications for PPP1R1B blocking peptide: Blocking (BL)
• Application Notes: Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
• Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PPP1R1B blocking peptide

The peptide is used to block Anti-DARPP32 reactivity.

NCBI and Uniprot Product Information

• NCBI GI #: 23831292
• NCBI GeneID: 84152
• UniProt Accession #: Q9UD71; Q547V9; Q547W0; Q9H7G1
• Molecular Weight: 18,738 Da
• NCBI Official Full Name: Protein phosphatase 1 regulatory subunit 1B
• NCBI Official Synonym Full Names: protein phosphatase 1 regulatory inhibitor subunit 1B
• NCBI Official Symbol: PPP1R1B
• NCBI Official Synonym Symbols: DARPP32; DARPP-32
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 1B
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 1B
• Protein Family: Protein phosphatase 1 regulatory
• UniProt Gene Name: PPP1R1B
• UniProt Synonym Gene Names: DARPP32
• UniProt Entry Name: PPR1B_HUMAN

NCBI Description

This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

DARPP-32: a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32. Two alternatively-spliced isoforms have been described.

Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: cell soma; cytoplasm; cytosol; nucleus

Molecular Function: D1 dopamine receptor binding; D2 dopamine receptor binding; D3 dopamine receptor binding; D4 dopamine receptor binding; D5 dopamine receptor binding; protein binding; protein kinase inhibitor activity; protein phosphatase inhibitor activity; protein phosphatase type 1 regulator activity; type 1 serine/threonine specific protein phosphatase inhibitor activity

Biological Process: negative regulation of catalytic activity; negative regulation of female receptivity; regulation of catalytic activity; response to amphetamine; signal transduction; transcription, DNA-dependent; visual learning