Acinus Peptide | ACIN1 peptide

Acinus (AAE7, ACN1, AC NON-UTILIZING 1, ACYL-ACTIVATING ENZYME 7, AT3G16910.1, Apoptotic Chromatin condensation inducer in nucleus) (Control Peptide)

Cat. Num. AAA633922

• Gene Names: ACIN1; ACN; ACINUS; fSAP152
• Purity: Purified; Purified 60-70%
• Synonyms: Acinus; Acinus (AAE7; ACN1; AC NON-UTILIZING 1; ACYL-ACTIVATING ENZYME 7; AT3G16910.1; Apoptotic Chromatin condensation inducer in nucleus) (Control Peptide); ACIN1 peptide

• Host: Synthetic peptide
• Purity/Purification: Purified; Purified 60-70%
• Form/Format: Supplied as a lyophilized powder.

• Application Notes: Suitable for use as control peptide in ELISA and Western Blot.
• Positive Control: K562 cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for ACIN1 peptide

Peptide (DTSENRPENDVPEPP) corresponding to amino acids 775 to 789 of human AcinusL, 48 to 62 of human AcinusS', or 17 to 31 of human AcinusS, which differ from those of mouse Acinus by one amino acid (2). For use with cat. no. A0600-02.

Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE (1). A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS' (2).

Product Categories/Family for ACIN1 peptide

Molecular Biology; MB-Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 259906024
• NCBI GeneID: 22985
• NCBI Accession #: NP_001158289.1
• NCBI GenBank Nucleotide #: NM_001164817.1
• UniProt Accession #: Q9UKV3; O75158; Q9UG91; Q9UKV1; Q9UKV2; B2RTT4; D3DS45
• Molecular Weight: 151,862 Da
• NCBI Official Full Name: apoptotic chromatin condensation inducer in the nucleus isoform 5
• NCBI Official Synonym Full Names: apoptotic chromatin condensation inducer 1
• NCBI Official Symbol: ACIN1
• NCBI Official Synonym Symbols: ACN; ACINUS; fSAP152
• NCBI Protein Information: apoptotic chromatin condensation inducer in the nucleus; functional spliceosome-associated protein 152
• UniProt Protein Name: Apoptotic chromatin condensation inducer in the nucleus
• Protein Family: Apoptotic chromatin condensation inducer in the nucleus
• UniProt Gene Name: ACIN1
• UniProt Synonym Gene Names: ACINUS; KIAA0670; Acinus
• UniProt Entry Name: ACINU_HUMAN

NCBI Description

Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. This gene encodes a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA fragmentation. This protein has also been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) that is deposited at splice junctions on mRNAs, as a consequence of pre-mRNA splicing. It may thus be involved in mRNA metabolism associated with splicing. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

acinus: an ubiquitous RNA-binding protein. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC is composed of ACIN1, CASC3, DDX48, MAGOH, PNN, RBM8A, RNPS1, SAP18 and THOC4. Forms heterodimers with RNPS1. Found in a heterotrimeric complex with ACIN1, RNPS1 and SAP18. Interacts with API5. Phosphorylation by SRPK2 redistributes it from the nuclear speckles to the nucleoplasm, and up-regulates its stimulatory effect on cyclin A1. Activated by proteolytic cleavage. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells. Four alternatively spliced human isoforms have been described.

Protein type: Apoptosis; RNA splicing; Spliceosome; RNA-binding

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleolus; nuclear speck; nucleus; cytosol

Molecular Function: protein binding; enzyme binding; nucleic acid binding; ATPase activity; nucleotide binding

Biological Process: positive regulation of monocyte differentiation; negative regulation of nuclear mRNA splicing, via spliceosome; positive regulation of apoptosis; apoptosis; RNA splicing; erythrocyte differentiation; apoptotic chromosome condensation; mRNA processing; cell structure disassembly during apoptosis