ZNF423 sirna

ZNF423 siRNA (Mouse)

Cat. Num. AAA8235193

• Gene Names: Zfp423; Roaz; Ebfaz; nur12; Zfp104; Znf423; ataxia1; mKIAA0760
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZNF423; ZNF423 siRNA (Mouse); EBFAZ; KIAA0760; NUR12; OAZ; ZFP423; Zinc finger protein 423; Early B-cell factor-associated zinc finger protein; Olf1/EBF-associated zinc finger protein; Smad- and Olf-interacting zinc finger protein; ZNF423 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ZNF423 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1292

• Applicable Applications for ZNF423 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ZNF423 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZNF423 sirna

siRNA to inhibit ZNF423 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 46359077
• NCBI GeneID: 94187
• NCBI Accession #: NP_201584.2
• NCBI GenBank Nucleotide #: NM_033327.2
• UniProt Accession #: Q80TS5; Q6PCP2; Q6X497; Q8CIQ1; Q9ESD2; B2RSW4
• Molecular Weight: 143,090 Da
• NCBI Official Full Name: zinc finger protein 423 isoform 1
• NCBI Official Synonym Full Names: zinc finger protein 423
• NCBI Official Symbol: Zfp423
• NCBI Official Synonym Symbols: Roaz; Ebfaz; nur12; Zfp104; Znf423; ataxia1; mKIAA0760
• NCBI Protein Information: zinc finger protein 423
• UniProt Protein Name: Zinc finger protein 423
• Protein Family: Zinc finger protein
• UniProt Gene Name: Znf423
• UniProt Synonym Gene Names: Ebfaz; Kiaa0760; Nur12; Oaz; Zfp423
• UniProt Entry Name: ZN423_MOUSE

Uniprot Description

ZNF423: Transcription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved in terminal olfactory receptor neurons differentiation; this interaction preventing EBF1 to bind DNA and activate olfactory-specific genes. Involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons. Controls proliferation and differentiation of neural precursors in cerebellar vermis formation. Defects in ZNF423 can be a cause of nephronophthisis-related ciliopathies (NPHP-RC), a group of recessive diseases that affect kidney, retina and brain. ZNF423 mutations have been found in patients with nephronophthisis, cerebellar vermis hypoplasia and situs inversus, and Joubert syndrome. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding

Cellular Component: nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; nucleic acid binding; protein heterodimerization activity; sequence-specific DNA binding; metal ion binding

Biological Process: nervous system development; Notch signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription, DNA-dependent; negative regulation of transcription, DNA-dependent; cell differentiation; positive regulation of BMP signaling pathway