XIAP sirna

XIAP siRNA (Mouse)

Cat. Num. AAA8239905

• Gene Names: Xiap; Aipa; Api3; IAP3; MIHA; Birc4; ILP-1; 1110015C02Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: XIAP; XIAP siRNA (Mouse); AIPA; API3; BIRC4; MIHA; E3 ubiquitin-protein ligase XIAP; Baculoviral IAP repeat-containing protein 4; IAP homolog A; Inhibitor of apoptosis protein 3; IAP-3; mIAP-3; mIAP3; X-linked inhibitor of apoptosis protein; X-linked IAP; XIAP sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: XIAP siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 496

• Applicable Applications for XIAP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse XIAP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for XIAP sirna

siRNA to inhibit XIAP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 675022741
• NCBI GeneID: 11798
• NCBI Accession #: NP_001288568.1
• NCBI GenBank Nucleotide #: NM_001301639.1
• UniProt Accession #: Q60989; O08865; A2BGY6
• Molecular Weight: 56,079 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase XIAP
• NCBI Official Synonym Full Names: X-linked inhibitor of apoptosis
• NCBI Official Symbol: Xiap
• NCBI Official Synonym Symbols: Aipa; Api3; IAP3; MIHA; Birc4; ILP-1; 1110015C02Rik
• NCBI Protein Information: E3 ubiquitin-protein ligase XIAP
• UniProt Protein Name: E3 ubiquitin-protein ligase XIAP
• Protein Family: XIAP-associated factor
• UniProt Gene Name: Xiap
• UniProt Synonym Gene Names: Aipa; Api3; Birc4; Miha; IAP-3; mIAP-3; mIAP3; X-linked IAP
• UniProt Entry Name: XIAP_MOUSE

NCBI Description

The protein encoded by this gene is a member of the inhibitor of apoptosis (IAP) family of proteins. While first identified for its role in blocking apoptosis, this protein modulates many other signaling processes including nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) pathways and inflammatory responses. This protein blocks apoptosis by binding and inhibiting target caspases after they have been activated. Binding occurs to some, but not all, caspases. This protein has several conserved regions, including baculoviral IAP repeat (BIR) motifs and a RING finger E3 ligase domain. In humans, mutations in this gene are linked to immunodeficiency in X-linked lymphoproliferative syndrome type-2 (XLP-2). A pseudogene of this gene is found on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]

Uniprot Description

XIAP: an apoptotic suppressor protein. Inhibist apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. Inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. Inhibits cell-death proteases, caspase-3, caspase-7, and perhaps caspase-9.

Protein type: Ligase; Apoptosis; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-

Cellular Component: protein complex; perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; protease binding; zinc ion binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; caspase inhibitor activity; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: neuron apoptosis; Wnt receptor signaling pathway; positive regulation of protein ubiquitination; apoptosis; cytokinesis; protein ubiquitination; negative regulation of caspase activity; negative regulation of neuron apoptosis; negative regulation of apoptosis