WNT7A sirna

WNT7A siRNA (Mouse)

Cat. Num. AAA8224337

• Gene Names: Wnt7a; px; tw; Wnt-7a; AI849442
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WNT7A; WNT7A siRNA (Mouse); WNT-7A; Protein Wnt-7a; WNT7A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: WNT7A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 349

• Applicable Applications for WNT7A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WNT7A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WNT7A sirna

siRNA to inhibit WNT7A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31543960
• NCBI GeneID: 22421
• NCBI Accession #: NP_033553.2
• NCBI GenBank Nucleotide #: NM_009527.3
• UniProt Accession #: P24383; Q80VH3; Q9DBY3
• Molecular Weight: 38,974 Da
• NCBI Official Full Name: protein Wnt-7a
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 7A
• NCBI Official Symbol: Wnt7a
• NCBI Official Synonym Symbols: px; tw; Wnt-7a; AI849442
• NCBI Protein Information: protein Wnt-7a
• UniProt Protein Name: Protein Wnt-7a
• UniProt Gene Name: Wnt7a
• UniProt Synonym Gene Names: Wnt-7a
• UniProt Entry Name: WNT7A_MOUSE

Uniprot Description

WNT7A: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts. Defects in WNT7A are the cause of limb pelvis hypoplasia aplasia syndrome (LPHAS). A syndrome of severe deficiency of the extremities due to hypo- or aplasia of one or more long bones of one or more limbs. Pelvic manifestations include hip dislocation, hypoplastic iliac bone and aplastic pubic bones. Thoracic deformity, unusual facies and genitourinary anomalies can be present. Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS); also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia. Belongs to the Wnt family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; cell; extracellular region

Molecular Function: protein binding; frizzled binding; cytokine activity; receptor agonist activity; receptor binding

Biological Process: hindlimb morphogenesis; somatic stem cell maintenance; positive regulation of epithelial cell proliferation involved in wound healing; positive regulation of transcription, DNA-dependent; multicellular organismal development; Wnt receptor signaling pathway through beta-catenin; uterus development; cell-cell signaling; regulation of axon diameter; synapse organization and biogenesis; somatic stem cell division; chondrocyte differentiation; satellite cell activation; cartilage condensation; embryonic limb morphogenesis; cell fate commitment; skin morphogenesis; asymmetric protein localization; positive regulation of synaptogenesis; organ morphogenesis; response to estrogen stimulus; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of apoptosis; embryonic forelimb morphogenesis; limb development; cell proliferation in forebrain; neurotransmitter secretion; positive regulation of JNK cascade; palate development; regulation of axonogenesis; signal transduction; embryonic hindlimb morphogenesis; negative regulation of neurogenesis; forelimb morphogenesis; central nervous system vasculogenesis; positive regulation of cell proliferation; angiogenesis; fallopian tube development; Wnt receptor signaling pathway; embryonic axis specification; satellite cell compartment self-renewal involved in skeletal muscle regeneration; cerebellar granule cell differentiation; regulation of cell proliferation; stem cell development; dorsal/ventral pattern formation; establishment of cell polarity; skeletal morphogenesis; cartilage development; reproductive structure development