WNT4 sirna

WNT4 siRNA (Mouse)

Cat. Num. AAA8221144

• Gene Names: Wnt4; Wnt-4
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WNT4; WNT4 siRNA (Mouse); WNT-4; Protein Wnt-4; WNT4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: WNT4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 351

• Applicable Applications for WNT4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WNT4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WNT4 sirna

siRNA to inhibit WNT4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6678595
• NCBI GeneID: 22417
• NCBI Accession #: NP_033549.1
• NCBI GenBank Nucleotide #: NM_009523.2
• UniProt Accession #: P22724
• Molecular Weight: 39,050 Da
• NCBI Official Full Name: protein Wnt-4
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 4
• NCBI Official Symbol: Wnt4
• NCBI Official Synonym Symbols: Wnt-4
• NCBI Protein Information: protein Wnt-4
• UniProt Protein Name: Protein Wnt-4
• UniProt Gene Name: Wnt4
• UniProt Synonym Gene Names: Wnt-4
• UniProt Entry Name: WNT4_MOUSE

Uniprot Description

WNT4: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Overexpression may be associated with abnormal proliferation in human breast tissue. Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome); also called Mayer- Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls. Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL); also known as SERKAL syndrome. Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL). Belongs to the Wnt family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; cell surface; cytoplasm; extracellular region

Molecular Function: protein binding; frizzled binding; transcription corepressor activity; receptor binding

Biological Process: anatomical structure development; negative regulation of fibroblast growth factor receptor signaling pathway; multicellular organismal development; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; T cell differentiation in the thymus; thyroid stimulating hormone secreting cell differentiation; Wnt receptor signaling pathway through beta-catenin; androgen biosynthetic process; signal transduction; somatotropin secreting cell differentiation; neuron differentiation; regulation of cell-cell adhesion; cell-cell signaling; positive regulation of focal adhesion formation; positive regulation of aldosterone biosynthetic process; positive regulation of stress fiber formation; embryonic epithelial tube formation; mesonephros development; cell differentiation; negative regulation of cell migration; female gonad development; Wnt receptor signaling pathway; cell fate commitment; positive regulation of meiosis; male gonad development; immature T cell proliferation in the thymus; cellular response to starvation; positive regulation of bone mineralization; positive regulation of osteoblast differentiation; negative regulation of cell differentiation; protein palmitoylation; branching morphogenesis of a tube; ureteric bud branching; gamete generation; hormone metabolic process; smooth muscle cell differentiation; female sex determination; negative regulation of transcription, DNA-dependent; tube morphogenesis; metanephros development; sex differentiation; oocyte development; positive regulation of GTPase activity