WNT11 sirna

WNT11 siRNA (Mouse)

Cat. Num. AAA8223595

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WNT11; WNT11 siRNA (Mouse); WNT-11; Protein Wnt-11; WNT11 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: WNT11 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 354

• Applicable Applications for WNT11 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WNT11 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WNT11 sirna

siRNA to inhibit WNT11 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 553727152
• NCBI GeneID: 22411
• NCBI Accession #: NP_001272721.1
• NCBI GenBank Nucleotide #: NM_001285792.1
• UniProt Accession #: P48615
• Molecular Weight: 39,135 Da
• NCBI Official Full Name: protein Wnt-11 isoform a
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 11
• NCBI Official Symbol: Wnt11
• NCBI Protein Information: protein Wnt-11
• UniProt Protein Name: Protein Wnt-11
• Protein Family: Protein
• UniProt Gene Name: Wnt11
• UniProt Synonym Gene Names: Wnt-11
• UniProt Entry Name: WNT11_MOUSE

Uniprot Description

WNT11: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; proteinaceous extracellular matrix; cytoplasm; extracellular region

Molecular Function: protein binding; frizzled binding; protein kinase activator activity; receptor binding

Biological Process: cell fate commitment; Wnt receptor signaling pathway; positive regulation of apoptosis; multicellular organismal development; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin; palate development; positive regulation of transforming growth factor-beta2 production; signal transduction; protein amino acid phosphorylation; bone mineralization; osteoblast differentiation; neuron differentiation; organ morphogenesis; cell-cell signaling; positive regulation of protein kinase activity; positive regulation of stress fiber formation; artery morphogenesis; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; kidney development; negative regulation of cell migration; negative regulation of apoptosis; positive regulation of cell migration