VANGL2 sirna

VANGL2 siRNA (Mouse)

Cat. Num. AAA8224313

• Gene Names: Vangl2; Lp; Lpp1; Ltap; stbm; ska17; Vang1l2; loop-tail; strabismus; ska<m17Jus>; C530001F03Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VANGL2; VANGL2 siRNA (Mouse); LPP1; LTAP; Vang-like protein 2; Loop-tail protein 1; Loop-tail-associated protein; Van Gogh-like protein 2; VANGL2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: VANGL2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 521

• Applicable Applications for VANGL2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse VANGL2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VANGL2 sirna

siRNA to inhibit VANGL2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 15723382
• NCBI GeneID: 93840
• NCBI Accession #: NP_277044.1
• NCBI GenBank Nucleotide #: NM_033509.3
• UniProt Accession #: Q91ZD4; Q923Z8
• Molecular Weight: 59,771 Da
• NCBI Official Full Name: vang-like protein 2
• NCBI Official Synonym Full Names: vang-like 2 (van gogh, Drosophila)
• NCBI Official Symbol: Vangl2
• NCBI Official Synonym Symbols: Lp; Lpp1; Ltap; stbm; ska17; Vang1l2; loop-tail; strabismus; ska<m17Jus>; C530001F03Rik
• NCBI Protein Information: vang-like protein 2
• UniProt Protein Name: Vang-like protein 2
• Protein Family: Vang-like protein
• UniProt Gene Name: Vangl2
• UniProt Synonym Gene Names: Lpp1; Ltap
• UniProt Entry Name: VANG2_MOUSE

Uniprot Description

VANGL2: Involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. Plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. Required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process. Interacts through its C-terminal region with the N- terminal half of DVL1, DVL2 and DVL3. The PDZ domain of DVL1, DVL2 and DVL3 is required for the interaction. Also interacts with the PDZ domains of MAGI3, SCRIB/SCRB1 and FZD3. Belongs to the Vang family.

Protein type: Membrane protein, multi-pass; Adaptor/scaffold; Membrane protein, integral

Cellular Component: membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane; stress fiber; integral to membrane; intracellular; intercellular junction; ER to Golgi transport vesicle; lateral plasma membrane

Molecular Function: protein binding

Biological Process: positive regulation of JNK activity; convergent extension involved in axis elongation; wound healing; somatic stem cell maintenance; multicellular organismal development; glomerulus development; Rho protein signal transduction; anterior/posterior pattern formation; apical protein localization; hair follicle development; sensory cilium biogenesis; somatic stem cell division; establishment of planar polarity; heart looping; Wnt receptor signaling pathway, planar cell polarity pathway; inner ear receptor cell development; convergent extension involved in neural plate elongation; regulation of Wnt receptor signaling pathway; digestive tract morphogenesis; patterning of blood vessels; establishment and/or maintenance of epithelial cell polarity; convergent extension involved in organogenesis; inner ear receptor stereocilium organization and biogenesis; regulation of actin cytoskeleton organization and biogenesis; establishment of body hair orientation; neural tube closure