USP15 sirna

USP15 siRNA (Mouse)

Cat. Num. AAA8238448

• Gene Names: Usp15; Gcap18; AI327321; 4921514G19Rik; E430033I05Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: USP15; USP15 siRNA (Mouse); KIAA0529; Ubiquitin carboxyl-terminal hydrolase 15; Deubiquitinating enzyme 15; Ubiquitin thioesterase 15; Ubiquitin-specific-processing protease 15; USP15 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: USP15 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 952

• Applicable Applications for USP15 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse USP15 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for USP15 sirna

siRNA to inhibit USP15 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 674651075
• NCBI GeneID: 14479
• NCBI Accession #: NP_001288557.1
• NCBI GenBank Nucleotide #: NM_001301628.1
• UniProt Accession #: Q8R5H1; Q3TGF5; Q3TTB2; Q3UL25; Q3UZH0; Q80TY6; Q80UK9
• Molecular Weight: 109,220 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 15 isoform 2
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 15
• NCBI Official Symbol: Usp15
• NCBI Official Synonym Symbols: Gcap18; AI327321; 4921514G19Rik; E430033I05Rik
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 15
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 15
• UniProt Gene Name: Usp15
• UniProt Synonym Gene Names: Kiaa0529
• UniProt Entry Name: UBP15_MOUSE

NCBI Description

The protein encoded by this gene is a member of the large ubiquitin specific protease (Usp) family of proteins. These proteins are known to cleave ubiquitin, and contain a conserved cysteine residue (Cys box) and two conserved histidine residues (His box) that are thought to form part of the active site of the protease. This protein has been shown to cleave both the ubiquitin-proline and the ubiquitin-methionine bonds in vitro. This protein is thought to regulate many cellular processes through its deubiquitination activity, including the transforming growth factor beta (TGF-beta) pathway. Cardiac-specific overexpression of the human ortholog of this gene in mice causes enlargement of the heart that is more pronounced in the atrium than in the ventricle. This gene has two pseudogenes on chromosome 14. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms.[provided by RefSeq, Aug 2014]

Uniprot Description

USP15: Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Ubiquitin conjugating system; EC 3.4.19.12; Ubiquitin-specific protease

Cellular Component: cytoplasm; nucleus

Molecular Function: peptidase activity; identical protein binding; hydrolase activity; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; SMAD binding; transforming growth factor beta receptor binding; catalytic activity; cysteine-type peptidase activity

Biological Process: BMP signaling pathway; ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; transforming growth factor beta receptor signaling pathway; proteolysis