UIMC1 sirna

UIMC1 siRNA (Mouse)

Cat. Num. AAA8209888

• Gene Names: Uimc1; RIP110; Rxrip110; 9430016E08Rik; D330018D10Rik; D630032M02Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UIMC1; UIMC1 siRNA (Mouse); RAP80; RIP110; RXRIP110; BRCA1-A complex subunit RAP80; Receptor-associated protein 80; Ubiquitin interaction motif-containing protein 1; UIMC1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: UIMC1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 446

• Applicable Applications for UIMC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse UIMC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UIMC1 sirna

siRNA to inhibit UIMC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 652698318
• NCBI GeneID: 20184
• NCBI Accession #: NP_001280589.1
• NCBI GenBank Nucleotide #: NM_001293660.1
• UniProt Accession #: Q5U5Q9; Q3TU77; Q60811; Q8C3Z8; Q8C719; Q8K1X7
• Molecular Weight: 50,382 Da
• NCBI Official Full Name: BRCA1-A complex subunit RAP80 isoform 2
• NCBI Official Synonym Full Names: ubiquitin interaction motif containing 1
• NCBI Official Symbol: Uimc1
• NCBI Official Synonym Symbols: RIP110; Rxrip110; 9430016E08Rik; D330018D10Rik; D630032M02Rik
• NCBI Protein Information: BRCA1-A complex subunit RAP80
• UniProt Protein Name: BRCA1-A complex subunit RAP80
• Protein Family: BRCA1-A complex
• UniProt Gene Name: Uimc1
• UniProt Synonym Gene Names: Rap80; Rip110; Rxrip110
• UniProt Entry Name: UIMC1_MOUSE

Uniprot Description

RAP80: Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. Interacts with TSP57. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with FAM175A/Abraxas. Interacts with ESR1, NR6A1 and UBE2I. Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis. Belongs to the RAP80 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Cellular Component: nucleus

Molecular Function: retinoid X receptor binding; histone binding

Biological Process: positive regulation of DNA repair; regulation of transcription, DNA-dependent; transcription, DNA-dependent; double-strand break repair; response to ionizing radiation; chromatin modification; G2/M transition DNA damage checkpoint; negative regulation of transcription, DNA-dependent; DNA repair; response to DNA damage stimulus