TTLL8 sirna

TTLL8 siRNA (Mouse)

Cat. Num. AAA8234040

• Gene Names: Ttll8; 4933401B17; 1700019P01Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TTLL8; TTLL8 siRNA (Mouse); Protein monoglycylase TTLL8; Tubulin--tyrosine ligase-like protein 8; TTLL8 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TTLL8 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 832

• Applicable Applications for TTLL8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TTLL8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TTLL8 sirna

siRNA to inhibit TTLL8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 295293121
• NCBI GeneID: 239591
• NCBI Accession #: NP_766406.2
• NCBI GenBank Nucleotide #: NM_172818.3
• UniProt Accession #: A4Q9F1; Q14B76; Q8C0N7
• Molecular Weight: 94,915 Da
• NCBI Official Full Name: protein monoglycylase TTLL8
• NCBI Official Synonym Full Names: tubulin tyrosine ligase-like family, member 8
• NCBI Official Symbol: Ttll8
• NCBI Official Synonym Symbols: 4933401B17; 1700019P01Rik
• NCBI Protein Information: protein monoglycylase TTLL8
• UniProt Protein Name: Protein monoglycylase TTLL8
• Protein Family: Protein monoglycylase
• UniProt Gene Name: Ttll8
• UniProt Entry Name: TTLL8_MOUSE

Uniprot Description

TTLL8: Monoglycylase which modifies both tubulin and non- tubulin proteins, generating side chains of glycine on the gamma- carboxyl groups of specific glutamate residues of target proteins. Monoglycylates tubulin, with a preference for alpha-tubulin toward beta-tubulin. Has the ability to modify non-tubulin proteins such as ANP32A, ANP32B, SET and NCL. Involved in the side-chain initiation step of the glycylation reaction by adding a single glycine chain to generate monoglycine side chains. Not involved in elongation step of the polyglycylation reaction.

Protein type: EC 6.3.2.-

Cellular Component: microtubule cytoskeleton; microtubule; cytoskeleton; cell projection; cytoplasm; axoneme; cilium

Molecular Function: coenzyme F420-2 alpha-glutamyl ligase activity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ribosomal S6-glutamic acid ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: protein polyglycylation; protein modification process; cilium biogenesis