TTC21B sirna

TTC21B siRNA (Mouse)

Cat. Num. AAA8212192

• Gene Names: Ttc21b; aln; Thm1; mKIAA1992; 2410066K11Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TTC21B; TTC21B siRNA (Mouse); KIAA1992; THM1; Tetratricopeptide repeat protein 21B; TPR repeat protein 21B; Tetratricopeptide repeat-containing hedgehog modulator 1; TTC21B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TTC21B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1315

• Applicable Applications for TTC21B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TTC21B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TTC21B sirna

siRNA to inhibit TTC21B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 114158711
• NCBI GeneID: 73668
• NCBI Accession #: NP_001041069.1
• NCBI GenBank Nucleotide #: NM_001047604.2
• UniProt Accession #: Q0HA38; Q3V1U7; Q69Z46
• Molecular Weight: 150,795 Da
• NCBI Official Full Name: tetratricopeptide repeat protein 21B isoform a
• NCBI Official Synonym Full Names: tetratricopeptide repeat domain 21B
• NCBI Official Symbol: Ttc21b
• NCBI Official Synonym Symbols: aln; Thm1; mKIAA1992; 2410066K11Rik
• NCBI Protein Information: tetratricopeptide repeat protein 21B
• UniProt Protein Name: Tetratricopeptide repeat protein 21B
• Protein Family: Tetratricopeptide repeat protein
• UniProt Gene Name: Ttc21b
• UniProt Synonym Gene Names: Kiaa1992; Thm1; TPR repeat protein 21B
• UniProt Entry Name: TT21B_MOUSE

Uniprot Description

TTC21B: May negatively modulate SHH signal transduction and may play a role in retrograde intraflagellar transport in cilia. Ciliary dysfunction leads to a broad spectrum of disorders, collectively termed ciliopathies. Overlapping clinical features include retinal degeneration, renal cystic disease, skeletal abnormalities, fibrosis of various organ, and a complex range of anatomical and functional defects of the central and peripheral nervous system. The ciliopathy range of diseases includes Meckel-Gruber syndrome, Bardet-Biedl syndrome, Joubert syndrome, nephronophtisis, Senior-Loken syndrome, and Jeune asphyxiating thoracic dystrophy among others. TTC21B is causally associated with diverse ciliopathies, and also acts as a modifier gene across the ciliopathy spectrum. TTC21B mutations interact in trans with mutations in other ciliopathy-causing genes and contribute to disease manifestation and severity. Defects in TTC21B are the cause of nephronophthisis type 12 (NPHP12). NPHP12 is an autosomal recessive disorder resulting in end-stage renal disease. It is a progressive tubulo-interstitial kidney disorder histologically characterized by modifications of the tubules with thickening of the basement membrane, interstitial fibrosis and, in the advanced stages, medullary cysts. Some patients manifest extra-renal features including retinal, skeletal and central nervous system defects. Defects in TTC21B are the cause of asphyxiating thoracic dystrophy type 4 (ATD4). ATD4 is an autosomal recessive chondrodysplasia characterized by a severely constricted thoracic cage, short-limbed short stature, and polydactyly. It often leads to death in infancy because of respiratory insufficiency. Retinal degeneration, cystic renal disease and hepatic disease can be present in affected individuals who survive early childhood. Defects in TTC21B may be a cause of Bardet-Biedl syndrome (BBS). A syndrome characterized by usually severe pigmentary retinopathy, early-onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. Bardet-Biedl syndrome inheritance is autosomal recessive, but three mutated alleles (two at one locus, and a third at a second locus) may be required for clinical manifestation of some forms of the disease. Defects in TTC21B may be a cause of Joubert syndrome (JBTS). A disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease. Belongs to the TTC21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Cellular Component: cell projection; cytoskeleton; cell; nuclear chromatin; cytoplasm; intracellular; cilium

Biological Process: regulation of transcription from RNA polymerase II promoter; smoothened signaling pathway; ventricular system development; forebrain dorsal/ventral pattern formation; regulation of smoothened signaling pathway