TFAP2A sirna

TFAP2A siRNA (Mouse)

Cat. Num. AAA8235199

• Gene Names: Tfap2a; Ap2; AP-2; Ap2tf; Tcfap2a; AP2alpha; Ap-2 (a)
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TFAP2A; TFAP2A siRNA (Mouse); AP2TF; TCFAP2A; Transcription factor AP-2-alpha; AP2-alpha; AP-2 transcription factor; Activating enhancer-binding protein 2-alpha; Activator protein 2; AP-2; TFAP2A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TFAP2A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 437

• Applicable Applications for TFAP2A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TFAP2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TFAP2A sirna

siRNA to inhibit TFAP2A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 676260287
• NCBI GeneID: 21418
• NCBI Accession #: NP_001288603.1
• NCBI GenBank Nucleotide #: NM_001301674.1
• UniProt Accession #: P34056; Q60740; Q60741; Q60742; Q60743; Q62067; Q62068; Q62069; Q91VX0; Q9CRY4
• Molecular Weight: 50,900 Da
• NCBI Official Full Name: transcription factor AP-2-alpha isoform 1
• NCBI Official Synonym Full Names: transcription factor AP-2, alpha
• NCBI Official Symbol: Tfap2a
• NCBI Official Synonym Symbols: Ap2; AP-2; Ap2tf; Tcfap2a; AP2alpha; Ap-2 (a)
• NCBI Protein Information: transcription factor AP-2-alpha
• UniProt Protein Name: Transcription factor AP-2-alpha
• Protein Family: Transcription factor
• UniProt Gene Name: Tfap2a
• UniProt Synonym Gene Names: Ap2tf; Tcfap2a; AP2-alpha; AP-2
• UniProt Entry Name: AP2A_MOUSE

NCBI Description

This gene is a member of the activator protein 2 (AP-2) transcription factor family. The protein encoded by this gene can act as both an activator and repressor of gene transcription, and plays an important role in early embryogenesis, specifically in cranial development. This protein forms both homodimers and heterodimers, and binds to a GC-rich consensus sequence found in some promoters and enhancers. Disruption of this gene causes perinatal death, with neural tube, craniofacial, and limb mesenchyme defects. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms. [provided by RefSeq, Sep 2014]

Uniprot Description

AP-2 alpha: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis. Interacts with KCTD1; this interaction represses transcription activation. Interacts (via C-terminus) with CITED2 (via C-terminus); the interaction stimulates TFAP2A- transcriptional activation. Interacts (via N-terminus) with EP300 (via N-terminus); the interaction requires CITED2. Belongs to the AP-2 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Cellular Component: nucleoplasm; Golgi apparatus; centrosome; transcription factor complex; intracellular membrane-bound organelle; cytoplasm; cytosol; nucleus

Molecular Function: protein dimerization activity; protein binding; DNA binding; sequence-specific DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; embryonic forelimb morphogenesis; transcription from RNA polymerase II promoter; skin development; neural crest cell development; regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; palate development; embryonic pattern specification; forelimb morphogenesis; negative regulation of cell proliferation; sympathetic nervous system development; sensory perception of sound; regulation of transcription, DNA-dependent; oculomotor nerve formation; epidermis morphogenesis; regulation of cell differentiation; positive regulation of neuron apoptosis; negative regulation of neuron apoptosis; kidney development; trigeminal nerve development; inner ear morphogenesis; transcription, DNA-dependent; anterior neuropore closure; embryonic cranial skeleton morphogenesis; positive regulation of bone mineralization; regulation of transcription from RNA polymerase II promoter; embryonic body morphogenesis; skeletal morphogenesis; forebrain neuron development; neural tube closure; lens morphogenesis in camera-type eye; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; positive regulation of cell migration; negative regulation of apoptosis