TEX14 sirna

TEX14 siRNA (Mouse)

Cat. Num. AAA8239896

• Gene Names: Tex14; C85585
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TEX14; TEX14 siRNA (Mouse); Inactive serine/threonine-protein kinase TEX14; Testis-expressed sequence 14; Testis-expressed sequence 14 protein; TEX14 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TEX14 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1462

• Applicable Applications for TEX14 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TEX14 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TEX14 sirna

siRNA to inhibit TEX14 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 313151212
• NCBI GeneID: 83560
• NCBI Accession #: NP_001186222.1
• NCBI GenBank Nucleotide #: NM_001199293.1
• UniProt Accession #: Q7M6U3; Q3UT36; Q5NC10; Q5NC11; Q8CGK1; Q8CGK2; Q99MV8; B2KGL0
• Molecular Weight: 138,851 Da
• NCBI Official Full Name: inactive serine/threonine-protein kinase TEX14 isoform a
• NCBI Official Synonym Full Names: testis expressed gene 14
• NCBI Official Symbol: Tex14
• NCBI Official Synonym Symbols: C85585
• NCBI Protein Information: inactive serine/threonine-protein kinase TEX14
• UniProt Protein Name: Inactive serine/threonine-protein kinase TEX14
• Protein Family: Inactive serine/threonine-protein kinase
• UniProt Gene Name: Tex14
• UniProt Entry Name: TEX14_MOUSE

Uniprot Description

TEX14: Required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges. Also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Has no protein kinase activity in vitro. Belongs to the protein kinase superfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Cancer Testis Antigen (CTA); Protein kinase, Other; Other group; NKF5 family

Cellular Component: kinetochore; intercellular bridge; cell; cytoplasm; chromosome; midbody; chromosome, pericentric region

Molecular Function: protein binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: mitosis; cell division; mitotic cell cycle spindle assembly checkpoint; intercellular bridge organization and biogenesis; mitotic sister chromatid separation; negative regulation of protein binding; male meiosis; cell cycle; attachment of spindle microtubules to kinetochore; protein amino acid phosphorylation; negative regulation of cytokinesis