Human Dual Specificity Phosphatase 1 (DUSP1) ELISA Kit | DUSP1 elisa kit

Human Dual Specificity Phosphatase 1 (DUSP1) ELISA Kit

Cat. Num. AAA2022461

• Gene Names: DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10
• Reactivity: Human
• Synonyms: Dual Specificity Phosphatase 1 (DUSP1); Human Dual Specificity Phosphatase 1 (DUSP1) ELISA Kit; CL100; HVH1; MKP1; PTPN10; Mitogen-activated protein kinase phosphatase 1; Protein-tyrosine phosphatase CL100; Dual specificity protein phosphatase hVH1; DUSP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dual Specificity Phosphatase 1 (DUSP1).; No significant cross-reactivity or interference between Dual Specificity Phosphatase 1 (DUSP1) and analogues was observed.
• Sequence Length: 367

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 31.25-2000pg/mL
• Sensitivity: < 12.2pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dual Specificity Phosphatase 1 (DUSP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dual Specificity Phosphatase 1 (DUSP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DUSP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Specificity Phosphatase 1 (DUSP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Specificity Phosphatase 1 (DUSP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Specificity Phosphatase 1 (DUSP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dual Specificity Phosphatase 1 (DUSP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for DUSP1 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 18490273
• NCBI GeneID: 1843
• UniProt Accession #: P28562; Q2V508; D3DQL9
• Molecular Weight: 39,298 Da
• NCBI Official Full Name: Dual specificity phosphatase 1
• NCBI Official Synonym Full Names: dual specificity phosphatase 1
• NCBI Official Symbol: DUSP1
• NCBI Official Synonym Symbols: HVH1; MKP1; CL100; MKP-1; PTPN10
• NCBI Protein Information: dual specificity protein phosphatase 1
• UniProt Protein Name: Dual specificity protein phosphatase 1
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP1
• UniProt Synonym Gene Names: CL100; MKP1; PTPN10; VH1; MAP kinase phosphatase 1; MKP-1
• UniProt Entry Name: DUS1_HUMAN

NCBI Description

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

MKP-1: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP1 specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation. It is induced in human skin fibroblasts by oxidative/heat stress and growth factors. Contains 1 rhodanese domain.

Protein type: Protein phosphatase, dual-specificity; EC 3.1.3.16; Motility/polarity/chemotaxis; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: cytoplasm; nucleus

Molecular Function: protein tyrosine/threonine phosphatase activity; protein binding; non-membrane spanning protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: negative regulation of MAP kinase activity; response to light stimulus; response to cAMP; response to retinoic acid; positive regulation of apoptosis; negative regulation of MAPKKK cascade; response to glucocorticoid stimulus; negative regulation of meiotic cell cycle; response to testosterone stimulus; protein amino acid dephosphorylation; response to estradiol stimulus; cellular response to hormone stimulus; regulation of apoptosis; response to hydrogen peroxide; endoderm formation; response to oxidative stress; response to calcium ion; inactivation of MAPK activity; negative regulation of apoptosis