TAOK3 sirna

TAOK3 siRNA (Mouse)

Cat. Num. AAA8228152

• Gene Names: Taok3; A130052D22; 2900006A08Rik; A430105I05Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TAOK3; TAOK3 siRNA (Mouse); Serine/threonine-protein kinase TAO3; Thousand and one amino acid protein 3; TAOK3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TAOK3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 898

• Applicable Applications for TAOK3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TAOK3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TAOK3 sirna

siRNA to inhibit TAOK3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 124486801
• NCBI GeneID: 330177
• NCBI Accession #: NP_001074777.1
• NCBI GenBank Nucleotide #: NM_001081308.2
• UniProt Accession #: Q8BYC6; Q3V3B3
• Molecular Weight: 105,336 Da
• NCBI Official Full Name: serine/threonine-protein kinase TAO3
• NCBI Official Synonym Full Names: TAO kinase 3
• NCBI Official Symbol: Taok3
• NCBI Official Synonym Symbols: A130052D22; 2900006A08Rik; A430105I05Rik
• NCBI Protein Information: serine/threonine-protein kinase TAO3
• UniProt Protein Name: Serine/threonine-protein kinase TAO3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Taok3
• UniProt Entry Name: TAOK3_MOUSE

Uniprot Description

TAO3: Serine/threonine-protein kinase that acts as a regulator of the p38/MAPK14 stress-activated MAPK cascade and of the MAPK8/JNK cascade. Acts as an activator of the p38/MAPK14 stress- activated MAPK cascade. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Inhibits basal activity of MAPK8/JNK cascade and diminishes its activation in response epidermal growth factor (EGF). Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Protein type: Kinase, protein; Protein kinase, STE; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; TAO subfamily

Cellular Component: cytoplasm; intracellular

Molecular Function: transferase activity; protein serine/threonine kinase activity; MAP kinase kinase kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: positive regulation of JNK activity; negative regulation of JNK cascade; activation of MAPKK activity; protein amino acid autophosphorylation; MAPKKK cascade; positive regulation of JNK cascade; DNA repair; protein amino acid phosphorylation; positive regulation of stress-activated MAPK cascade; mitotic cell cycle G2/M transition DNA damage checkpoint; response to stress; phosphorylation; response to DNA damage stimulus